Influenza V 14-deu - virion\serion

More documents

Recommendations

Info

$Aspergillus V 11-eng NEU - virion\serion$

$Echovirus - SL - virion\serion$

$Coxsackievirus - SL - virion\serion$

$Enterovirus - SL - virion\serion$

$SERION ELISAclassic - virion\serion$

$PRODUKTÜBERSICHT PRODUCT OVERVIEW - virion\serion$

$West Nile Virus V 1-deu - virion\serion$

$Bestellformular SERION ELISA classic / antigen - virion\serion$

$Newsletter Nr. 17, 2011 - virion\serion$

$Bestellformular SERION ELISA Einzelreagenzien - virion\serion$

$Institut Virion\Serion GmbH Friedrich-Bergius-Ring 19 | D - 97076 ...$

$SERION ELISA classic Cytomegalovirus IgG / IgM - virion\serion$

$SERION ELISA classic Measles Virus IgG / IgM - virion\serion$

$SERION ELISA classic Bordetella pertussis IgA / IgG ... - virion\serion$

Pos: 34 /Ar bei tsanl eitungen ELISA cl assic/Gültig für all e D okumente/ELISA classic/T estauswertung/Testauswertung: Testgültig kei tskriterien @ 4\mod_1255348317229_18.doc @ 21432 @ 28 TEST EVALUATIONSERION ELISA classic Influenza A/B Virus IgA/IgG/IgMPos: 33 /Ar bei tsanl eitungen ELISA cl assic/Gültig für all e D okumente/ELISA classic/T estauswertung/Testauswertung: Ei n-Punkt-Quantifizi erung @ 4\mod_1255348099107_18.doc @ 21400 @ 28.1 Single-Point Quantification with the 4PL MethodOptimised assignment of extinction signals to quantitative values is guaranteed by usingnon-linear functions, which adjust a sigmoide curve without any further transformation toOD-values. Determination of antibody concentrations with the SERION ELISA classic iscarried out by the 4 parameter logistic-log-model (4 PL) which is ideal for exact curvefitting.It is based on the formula:ENEL ENOD = A +1 + eD - AB(C - ln Conc.)CS PTThe parameters A, B, C, and D are representative for the exact shape of the curve:1. lower asymptote parameter A2. slope of the curve parameter B3. turning point parameter C4. upper asymptote parameter DFor each lot the standard curve is evaluated by Institut Virion\Serion GmbH (Würzburg,Germany) in repeated test runs under optimal conditions. Time consuming and costintensive construction of the standard curve by the user is not necessary.For evaluation of antibody concentrations a lot specific standard curve as well as a lotspecific evaluation table is included with each SERION ELISA classic test kit. Theevaluation software SERION evaluate as well as the Microsoft ® Excel-based software toolSERION activity are available on request.To compensate for normal test variations and also for test run control a standard serum isused in each individual test run. For this control serum a reference value with a validityrange is determined by the quality control of the producer. Within this range a correctquantification of antibody concentration is ensured.english 15
Pos: 35 /Ar bei tsanl eitungen ELISA cl assic/Gültig für all e D okumente/ELISA classic/T estauswertung/Auswertung: Ü berschrift @ 0\mod_1180968661538_18.doc @ 970 @ 2Pos: 36 /Ar bei tsanl eitungen ELISA cl assic/Gültig für all e D okumente/ELISA classic/T estauswertung/Nichtautomatisierte Auswertung @ 4\mod_1255348011764_18.doc @ 21384 @ 38.2 Criteria of Validity- The substrate blank must be < 0.25 OD.- The negative control must produce a negative test result.- By use of quantitative SERION ELISA classic tests the mean OD-value (aftersubtraction of the substrate blank!) of the standard serum must be within the validityrange, which is given on the lot specific quality control certificate.- The variation of OD-values of the standard serum may not be higher than 20 %.If these criteria are not met, the test is not valid and must be repeated.8.3 Calculation SERION ELISA classic Influenza A/B Virus IgA/IgG/IgM8.3.1 Non-automated EvaluationFor the SERION ELISA classic test evaluation a lot-specific quality control certificate withstandard curve and an evaluation table is included in the test kit so that the obtained ODvalues may be assigned to the corresponding antibody activities. The substrate blank mustbe substracted from all OD values prior to evaluation.Pos: 37 /Ar bei tsanl eitungen ELISA cl assic/Gültig für mehrer e D okumente/T estauswertung/Testauswertung: M ethode 1 (für all e T ests auß er T etanus & Di phtherie) @ 5\mod_1255349684243_18.doc @ 21527 @sMethod 1: Qualitative EvaluationTo fix the cut-off ranges multiply the mean value of the measured standard OD with thenumerical data of the quality control certificate (see special case formulas), e.g.:OD = 0.502 x MW(STD) with upper cut-offOD = 0.352 x MW(STD) with lower cut-offIf the measured mean absorbance value of the standard serum is 0.64 OD, the range ofthe cut-off is in between 0.225-0.321 OD.english 16
Page 1 and 2: HerstellerManufacturerFabricantFabb
Page 3 and 4: Pos: 3 /Arbeitsanl eitung en ELISA
Page 7 and 8: Pos: 11 /Ar bei tsanl eitungen ELIS
Page 11 and 12: SERION ELISA classic Influenza A/B
Page 25 and 26: === Ende der Liste für T extmar ke
Page 29 and 30: 3 TEST PRINCIPLE SERION ELISA class
Page 31 and 32: 5 MATERIAL REQUIRED BUT NOT SUPPLIE
Page 39: Pos: 31 /Ar bei tsanl eitungen ELIS
Page 49 and 50: === Ende der Liste für T extmar ke
Page 53 and 54: 3 PRINCIPE DU TEST SERION ELISA cla
Page 55 and 56: 5 PRODUITS NÉCESSAIRES MAIS NON FO
Page 71 and 72: SERION ELISA classic Influenza A Vi
Page 77 and 78: 3 PRINCIPIO DEL TEST SERION ELISA c
Page 79 and 80: 5 MATERIALI NECESSARI, MA NON FORNI
Page 91 and 92:
Pos: 40 /Ar bei tsanl eitungen ELIS
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
=== Ende der Liste für T extmar ke
Page 99 and 100:
Pos: 3 /Arbeitsanl eitung en ELISA
Page 101 and 102:
3 ПРИНЦИП РАБОТЫ SERIO
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
7.5 Проведение анали
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
=== Ende der Liste für T extmar ke
Page 125 and 126:
AKCAGSTDPOSC/ONEGAPCAntikörper/ an
Page 127:
SERION ELISA classic102 Masern Viru
show all

Influenza V 14-deu - virion\serion

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?