Zusammenfassungen der Arbeitskreisbeiträge - Die DPG

whereas type A trichothecene producing species such as F. sporotrichioides and F.
langsethiae are more frequent. This shift in the spectrum of Fusarium species compared to
wheat does have consequences on contamination of grains with mycotoxins.
Artificial inoculation experiments under field-conditions were carried out to further
investigate the impact of different Fusarium species on pathology, grain infestation and
mycotoxin contamination in spring-barley. The appearance of the disease varied strongly
between different species and there was no general correlation detectable between the extent
of visual symptoms or grain-infestation and mycotoxin-contaminations in harvested grains.
PHYLOGENIE DER GATTUNG UROCYSTIS
Lotze-Engelhard, Sascha 1 ; Martin Kemler 2 , Dominik Begerow 1
1 Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie, AG Geobotanik,
Universitätsstraße 150, 44801 Bochum, Germany
2 Centre in Excellence in Tree Health Biotechnology Forestry and Agricultural Biotechnology
Institute (FABI), University of Pretoria, Pretoria 0002, South Africa
Contact: Sascha.Lotze-Engelhard@rub.de
Phylogeny of the genus Urocystis
The genus Urocystis Rabenhorst, which belongs to the Urocystales (Ustilaginomycotina),
comprises about 140 species and appears to be morphologically well defined. However,
molecular phylogenetic studies have shown that the monophyly could be questioned, and that
some other genera are at least closely related.
In the research summarised here, around 25% of the known species of the genus Urocystis
have been studied by molecular phylogenetic methods and presented in a comprehensive
analysis for the first time. The aim of this research was to elucidate the phylogeny of the
genus Urocystis. Therefore we used the genetic markers ITS, LSU and TEF1. The sequence
data formed the basis for the multi-gene analysis in which neighbour-joining, maximum
parsimony, maximum likelihood and bayesian interference were applied.
Overall, the results were concise, showing several monophyletic groups representing
Urocystis species on closely related host species. However, some genera that lack spore balls
of the Urocystis-type were mixed within the genus Urocystis and the backbone was not
always well resolved. As traditional genus description of spore balls did not support the
molecular phylogeny, we analysed the morphology of the spore balls in more detail. The
spores were categorized due to their number of fertile cells and checked against the
phylogenetic hypotheses obtained in the various analyses. With our data it is not possible to
resolve the phylogeny of the genus Urocystis completely, but it was clearly shown that the
genus Urocystis is paraphyletic in its present description because the genera Melanoxa,
Mundkurella, Ustacystis and Vankya cluster in the genus Urocystis.
Thus the new results raise far-reaching questions about the systematics of the whole complex
of genera Urocystis, Melanoxa, Mundkurella, Ustacystis and Vankya.
LIVING COLORS IN THE GRAY MOLD PATHOGEN: CODON-OPTIMIZED
GENES ENCODING GFP AND MCHERRY SHOWING BRIGHT FLUORESCENCE
IN BOTRYTIS CINEREA
Leroch, Michaela 1 ; Dennis Mernke 1 , Dieter Koppenhoefer 1 , Prisca Schneider 1 , Andreas
Mosbach 1 , Gunther Doehlemann 2 , Matthias Hahn 1
1
Department of Biology, University of Kaiserslautern, Kaiserslautern, Germany
2
Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
Contact: mleroch@rhrk.uni-kl.de
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