Chapter 1 | Introduction to Special Stains - Dako
Chapter 1 | Introduction to Special Stains - Dako
Chapter 1 | Introduction to Special Stains - Dako
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<strong>Introduction</strong> <strong>to</strong> <strong>Special</strong> <strong>Stains</strong><br />
Figure 1. H&E-stained section of skin<br />
with cutaneous blas<strong>to</strong>mycosis. High<br />
magnification (40x) view showing budding<br />
yeast with the inflamma<strong>to</strong>ry infiltrate.<br />
Hema<strong>to</strong>xylin stains the nuclei of cells blue<br />
<strong>to</strong> bluish-purple, and eosin stains other<br />
cellular elements in the tissues from pink<br />
<strong>to</strong> red (Figure courtesy: Sunil Badve, MD,<br />
FRCPath. Indiana University School of<br />
Medicine, Indianapolis, IN, USA).<br />
Figure 2. <strong>Special</strong> stained section of small<br />
intestine. The special stain mucicarmine is<br />
used for visualization of neutral epithelial<br />
mucins in small intestine. The mucins are<br />
stained rose <strong>to</strong> red, nuclei are blue/black<br />
(Weigert’s iron hema<strong>to</strong>xylin), and other<br />
tissue elements are yellow (metanil yellow<br />
or tartrazine).<br />
Table 1. 2009 biological stains certified by the Biological Stain Commission.<br />
Acid fuchsin, C.I. 42685<br />
Alcian blue 8 GX, C.I. 74240<br />
Alizarin red S, C.I. 58005<br />
Aniline blue WS, C.I. 42755<br />
Auramine O, C.I. 41000<br />
<strong>Introduction</strong> <strong>to</strong> <strong>Special</strong> <strong>Stains</strong><br />
2 | special stains and H & e special stains and H & e | 3<br />
Azocarmine B<br />
Azocarmine G, C.I. 50085<br />
Azure A, C.I. 52005<br />
Azure B, C.I. 52010<br />
Azure C, C.I. 52002<br />
Basic fuchsine, C.I. 42510<br />
Bismarck brown Y, C.I. 21000<br />
Brilliant cresyl blue, C.I. 51010<br />
Brilliant green, C.I. 42040<br />
Carmine, C.I. 75470<br />
Chlorazol black E, C.I. 30235<br />
Congo red, C.I. 22120<br />
Cresyl violet<br />
Crystal violet, C.I. 42555<br />
Darrow red<br />
Eosin B, C.I. 45400<br />
Eosin Y, C.I. 45380<br />
Erythrosin, C.I. 45430<br />
Ethyl eosin, C.I. 45386<br />
Ethyl green, C.I. 42590<br />
Fast green F C F, C.I. 42053<br />
Fluorescein Isothiocyanate<br />
Giemsa Stain 1902,<br />
modified in 1904.<br />
Hema<strong>to</strong>xylin, C.I. 75290<br />
(Bohmer 1865)<br />
Indigo carmine, C.I. 73015<br />
Janus green B, C.I. 11050<br />
Jenner stain 1899<br />
Light green SF, C.I. 42095<br />
Malachite green, C.I. 42000<br />
Martius yellow, C.I. 10315<br />
Methyl orange, C.I. 13025<br />
Methyl violet 2B, C.I. 42535<br />
Methylene blue<br />
Methylene blue, C.I. 52015<br />
Methylene violet<br />
(Bernthsen), C.I. 52041<br />
Neutral red, C.I. 50040<br />
Nigrosin, C.I. 50420<br />
Nile blue A, C.I. 51180<br />
Nuclear fast red, C.I. 60760<br />
Oil Red O, C.I. 26125<br />
Orange G, C.I. 16230<br />
Orange II, C.I. 15510<br />
Orcein<br />
Pararosaniline, C.I. 42500<br />
Phloxin B, C.I. 45410<br />
Protargol S<br />
Pyronine B, C.I. 45010<br />
Pyronine Y, C.I. 45005<br />
Resazurin<br />
Rose Bengal, C.I. 45435<br />
Safranine O, C.I. 50240<br />
Sudan black B, C.I. 26150<br />
Sudan III, C.I. 26100<br />
Sudan IV, C.I. 26105<br />
Tetrachrome stain (MacNeal)<br />
Thionine, C.I. 52000<br />
Toluidine blue, C.I. 52040<br />
Weigert 1878<br />
Wright stain (1908)
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