Chapter 1 | Introduction to Special Stains - Dako

Introduction to Special Stains Introduction to Special Stains
Figure 21. Small intestine stained with Alcian Blue/PAS, Dako Code AR169. This stain is used for the demonstration of neutral and acidic mucosubstances on the
Artisan Staining System. Alcian Blue pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. The
periodic acid-Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic
mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.
Figure 22a. Cell types seen in normal bone
marrow. Giemsa staining. (Figure from
Dako Education Guide, “The Illustrated
Guide to Bone Marrow Diagnosis,” 2nd
Edition (2009). Editors: Carlos Martin, MD,
and George L. Kumar, PhD).
Figure 22b. Giemsa staining. Atypical
mononuclear megakaryocyte in chronic
myeloid leukemia. (Figure from Dako
Education Guide, “The Illustrated Guide
to Bone Marrow Diagnosis,” 2nd Edition
(2009). Editors: Carlos Martin, MD, and
George L. Kumar, PhD).
22 | special stains and H & e special stains and H & e | 23
Erythroblast
Eosinophil
Myelocyte
Neutrophil
Normoblast
Plasma cell
Megakaryocyte