animal production - CSK Himachal Pradesh Agricultural University ...

In pox diseases the vesicular fluid, scabs scales are collected in 50% phosphate buffered glycerin (PBG).
4. Tongue, gum, snout epithelium
In Foot and Mouth Disease vesicular fluid is collected in sterile tubes and the vesicular epithelium from tongue, gum
or snout is collected in PBG. In Rinderprest the gum scrapings are collected in 50% PBG.
5. Uterine Biopsy
The material is collected with the help of biopsy catheter in PBG in viral infections like infections bovine rhinotracheitis,
bovine virus diarrhoea etc. affecting the genital organs.
6. Rectal swabs/stools
In enterovirus and rotavirus infections the rectal swabs are collected in TPB. The faecal samples are collected in
sterile containers.
7. Neurological diseases
In neurological diseases cerebrospinal fluid is collected with syringe by piercing between 3rd & 4th lumbar vertebrae.
In dead animals brain in PBG is collected. In early infections blood is heparin is collected.
Transport of specimens
Most of the viruses are sensitive to temperature and pH, therefore, these should be protected between collection and
processing time. The specimens after collection are kept in sterile transport medium. The pH should be 7.2 containing
anibiotics (crystalline penicillin 100 IU/ml, dihydrostreptomycin 200 mg/ml and mycostatin 100 ug/ml) to check bacterial or
fungal contamination. The specimen tubes should be sent over ice to the laboratory and in the laboratory specimens are
preserved at -20oC. Disease Histopathological Microbiological/
(In 10% formalin) Serological
Rabies Brain(Hippocampus Longitudinal int-
in canines, Cere- tact half in 50%
bellum in bovines) PBG
Rinderpest Pieces of intes- Whole blood in
tines, heart, EDTA,spleen,
mesentric lymph prescapular and
nodes and spleen. measentric lymph
nodes in PBG.
Foot & Tongue, heart, Vesicular fluid
Mouhth rumen pillars, or tongue scrap-
Disease pancreas ings in 50% PBG
(about 2.0 gram).
Malignant Liver, heart, Lung pieces,
Catarrhal lung, lymph lymph nodes and
Fever node, spleen, whole blood in
kidney, brain. EDTA.
Bovine Mesenteric lymph Blood, serum,
Viral nodes, intestine, measenteric lymph
Diarrhoea pancreas nodes, mucous
membrances of
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