animal production - CSK Himachal Pradesh Agricultural University ...

The pH is adjusted to 7.2 before autoclaving.
Tryptose Phosphate Broth(TPB)
It is used as a transport medium for nasal, eye, rectal swabs etc :
Tryptose 20.0 g
Dextrose 2.0 g
Sod. chloride 5.0 g
Di-sod. hydrogen phosphate 2.5 g
Distrilled water 1000 ml.
The pH is adjusted to 7.2 - 7.4 before autoclaving.
Antibiotics (pencillin @ 100 i.u./ml, dihydrostreptomycin @ 200 ug/ml and mycostatin 100 ug/ml) are added before
collection of swabs to check bacterial contamination.
Mycotic diseases
Mycotic infection eg Aspergillosis, blastomycosis, coccidiomycosis & cryptococcosis:
1. Affected tissues in sterile container without any preservativ for culture.
2. Affected tissue pieces for histopathological examination in 10% formalin.
Cutaneous mycotic infections:
Skin biopsy for culture and histopathological examination.
IMPORTANT PARASITIC DISEASES OF DOMESTIC ANIMAL AND BIRDS
Disease (Parasite) Symptoms/Lesions Treatment/Control
A. Cattle & Buffaloes
1. Fascioliosis Initially consti- Faecal examination
(Fasciola pation followed by is essential.
gigantica) diarrhoea, emacia- Zanil 10-12 mg/kg.B.W.
tion, dullness and Fasinex 12 mg/kg. B.W.
Weakness, oedema Infection may be avoided
of the throat. by grazing livestock on
higher grounds(snail-free)
2. Paramphi- Profuse fluidy Faecal examination is
stomiosis diarrhoea and essential.
(Amphistome weakness,dehy- Disease is caused by
spp.) dration immature states which
can be detected in fluidy
faeces.
Nilzan:33ml/50-100 kg.B.W.
Zanil:30 ml over 45 kg B.W.
30 ml upto 45 kg B.W.
3. Ascariasis Calf-hood diarr- Faecal examination is essential
(Toxocara hoea growth reta- Piperazine liquid: 30mg/calf
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