Fluensulfone ... - IR-4 Project
Fluensulfone ... - IR-4 Project
Fluensulfone ... - IR-4 Project
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SUMMARY<br />
PTRL West <strong>Project</strong> No. 1977W<br />
Report 2 Page 12<br />
A method for quantitation of MCW-2 and its metabolites in tomato, pepper, cucumber<br />
and melon was successfully validated. Control matrices were fortified at 0.01 mg/kg (the<br />
LOQ) and 0.10 mg/kg (10x LOQ). MCW-2 and its metabolites butene sulfonic acid (M-<br />
3627) and thiazole sulfonic acid (M-3625) were extracted from fortified tomatoes,<br />
pepper, cucumber and melon with acetonitrile:water. The extract was subjected to further<br />
cleanup involving an SPE cartridge cleanup. The final samples were analyzed by LC-<br />
MS/MS for quantitation of MCW-2, butene sulfonic acid and thiazole sulfonic acid. The<br />
limit of quantitation for all three analytes in all matrices is 0.01 ppm. The limit of<br />
detection was 0.45 ng/mL in solution.<br />
INTRODUCTION<br />
Makhteshim-Agan of North America, Inc. contracted with PTRL West, Inc. to conduct<br />
method development and validation of an analytical method for determination of MCW-2<br />
and its metabolites commonly named butene sulfonic acid and thiazole sulfonic acid in<br />
tomato, pepper, cucumber and melon.<br />
The study is designed to comply with OPPTS 860.1340 and SANCO/825/00 rev.7 dated<br />
17/03/04 guidelines. The study was initiated on January 15, 2010. This study was<br />
conducted from January 19 to May 13, 2010. The study was conducted at PTRL West,<br />
Inc., 625-B Alfred Nobel Drive, Hercules, CA 94547 under an approved protocol<br />
according to the US EPA FIFRA Good Laboratory Practice Standards, 40 CFR §160<br />
(Appendix A).