Fluensulfone ... - IR-4 Project

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PTRL West <strong>Project</strong> No. 1977W Report 2 Page 26 average percent recovery of M-3625 was 83% at 0.01 ppm and 79% at 0.10 ppm. The overall average percent recovery of M-3625 in melon was 81% with a standard deviation of 4%. The average percent recovery of M-3627 was 83% at 0.01 ppm and 76% at 0.10 ppm. The overall average percent recovery of M-3627 in melon was 79% with a standard deviation of 6%. The confirmation ions for MCW-2, M-3625 and M-3627 yielded acceptable recoveries at both fortification levels and the overall average recoveries were acceptable in melon. Representative control and fortified melon chromatograms are presented in Figures 38 through 40. CONCLUSIONS An analytical method was developed at validated for the analysis of MCW-2 and its metabolites thiazole sulfonic acid (M-3625) and butene sulfonic acid (M-3627) in tomatoes, peppers, cucumbers and melons. The method was validated at 0.01 ppm and 0.10 ppm, where no residues were detected in control matrices. The limit of quantitation for all three analytes in all matrices is 0.01 ppm. The limit of detection was 0.45 ng/mL in solution.
PTRL West <strong>Project</strong> No. 1977W Report 2 Page 27 Table I. Summary of Method Validation Recoveries of MCW-2, Butene Sulfonic Acid and Thiazole Sulfonic Acid in Tomato. Quantitation ions Fortification Level Percent Recovery Sample (ppm) MCW-2 M-3625 M-3627 Controls NA NA NA NA Fort 1A 0.01 100 78 97 Fort 1B 0.01 110 69 88 Fort 1C 0.01 100 78 98 Fort 1D 0.01 130 97 97 Fort 1E 0.01 90 95 96 Average 106 83 95 Standard Deviation 15 12 4 % Rel. Std. Dev. 14 14 4 Fort 2A 0.10 116 88 96 Fort 2B 0.10 94 89 97 Fort 2C 0.10 93 82 81 Fort 2D 0.10 100 72 102 Fort 2E 0.10 88 89 107 Average 98 84 97 Standard Deviation 11 7 10 % Rel. Std. Dev. 11 8 10 Overall Average 102 84 96 Overall Standard Deviation 13 9 7 Overall Average %RSD 13 11 7 NA =Not Applicable M-3625 = Thiazole sulfonic acid M-3627 = Butene sulfonic acid
Page 1 and 2: TITLE Method Validation of an Analy
Page 3 and 4: PTRL West Project No. 1977W Report
Page 5 and 6: STUDY PERSONNEL PTRL West Project N
Page 13 and 14: MATERIALS AND METHODS Reference Sub
Page 17 and 18: 3. Add 50 ml of ACN:HPLC water (1:1
Page 19 and 20: Injection Volume: 20 µL Solvent Sy
Page 21 and 22: Retention Time: Butene sulfonic aci
Page 25: PTRL West Project No. 1977W Report
Page 41: D. Impact on Study: Prepared By: No

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