Fluensulfone ... - IR-4 Project
Fluensulfone ... - IR-4 Project
Fluensulfone ... - IR-4 Project
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PTRL West <strong>Project</strong> No. 1977W<br />
Report 2 Page 23<br />
method validation, where the remaining were conducted with linear regression as<br />
calculated with Excel.<br />
The residue on treated matrices was calculated as follows:<br />
ppm MCW-2 (mg/kg) =<br />
ng/mL MCW - 2 x Init. Extract vol.<br />
(mL) x 0.001g/ng<br />
Sample Wt. (g)<br />
ppm butene sulfonic acid or thiazole sulfonic acid =<br />
g/mL<br />
acid<br />
x Eluate vol. (mL) x dilution factor x Init. Extract Vol. (mL)<br />
Aliquot vol.<br />
(mL) x Sample Wt. (g)<br />
Percent recovery for each analyte was calculated as follows:<br />
% MCW-2 Recovery =<br />
% BSA or %TSA Recovery =<br />
Residue Detected (ppm) - Average ppm Control<br />
Fortification<br />
Level (ppm)<br />
Residue Detected (ppm) - Average ppm Control<br />
Fortification<br />
Level(ppm)<br />
x Mol. Wt. Conversion Factor<br />
x 100<br />
x 100<br />
Validity of the MCW-2, butene sulfonic acid and thiazole sulfonic acid residue analytical<br />
methods was established by acceptable average recovery (70-110%) from fortified<br />
untreated control samples.<br />
An example calculation for the MCW-2 recovery in the tomato method validation (0.01<br />
ppm) is shown below:<br />
MCW-2 ppm =<br />
1.57 ng/mL x<br />
66 mL x<br />
10 g<br />
Percent Recovery MCW-2 =<br />
0.001<br />
0. 010 <br />
0.<br />
000<br />
0.01ppm<br />
= 0.010 ppm<br />
ppm<br />
x 100 = 100%<br />
An example calculation for the butene sulfonic acid recovery in cucumber method<br />
validation is shown below, where a similar calculation was conducted for thiazole<br />
sulfonic acid:
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