Full Journal - Journal of Cell and Molecular Biology - Haliç Üniversitesi

Figure 7: Effect of NaCI on activity of superoxidedismutase
in 4 culvitars of sesame (bars on data points are ±
SE of the mean).
Figure 9. (a) Protein Marker (14.5 –20l kD); (b) RT-46
(Control); (c) RT-46 (Heat stress); (d) RT-125 (Control); (e)
RT-125 (NaCl 30 mM); (f) RT-125 (Control); (g) RT-125
(NaCl 30 mM)
NaCl treatment inhibited growth of all the four
cultivars of S. indicum (Table 1). The responses to salt,
however, were genotype specific. Out of four
cultivars, cv. RT-125 showed maximum inhibition in
germination (Figure 1) and reduction in root and shoot
length in response to NaCl (Table 1). Root and shoot
length of RT-125 reduced by 46% and 66%
respectively whereas it was 34% and 50% over control
in RT-127 (Table 1).
Soluble proteins, total soluble sugars, sucrose and
proline contents increased in NaCl-treated seedlings of
all the four cultivars (Figures 2, 3, 4, 5). Soluble
protein levels were found higher in control seedlings
of RT-125 and RT-127 as compared to other two
Metabolic changes and protein patterns in salinity 35
Figure 8. Protein profile of 3 culvitars of Sesamum indicum
seedlings grown ih presence of different concentration
NaCI. Control: Seedlings grown in absence of NaCI
cultivars. Further increase in soluble proteins was 2 to
3 fold in RT-125 and RT-127 and less than two fold in
other two cultivars at 30 and 50mM NaCl (Figure 2).
The total soluble sugar and sucrose contents increased
in plants of all the four cultivars in presence of NaCl
(Figure 3, 4). The salinity-induced increase in total
soluble sugars and sucrose was two fold in RT-127 and
RT-46 under 30 and 50mM NaCl. Salt treatment
induced proline accumulation in all the four cultivars
(Figure 5). RT-54, RT-46 and RT-127 accumulated less
proline at 50mM NaCl as compared to RT-125 that
exhibited more than 2-fold rise in proline level.
Malondialdehyde (MDA) contents increased in
NaCl-treated seedlings of all the cultivars. However,
highest MDA content was noted in cv. RT-125 and the
least in RT-127 (Figure 6).
Control seedling of cv. RT-127 exhibited high
activity of SOD as compared to other cultivars (Figure
7). Seedlings of all four cultivars subjected to NaCl
stress exhibited increased activity of SOD. The
maximum increase (4- to 5-fold over control) in the
activity of SOD was recorded in cv. RT-127 followed
by 3- to 4-fold rise in RT-54 and RT-46 and 2- to 3-fold
in RT-125 at 30 and 50mM NaCl (Figure 7).
(ii) Electrophoretic patterns of proteins under NaCl
stress
All the cultivars of S. indicum subjected to 30 and 50
mM NaCl for five days exhibited alterations in their
protein profiles (Figure 8). The electrophoretically
separated protein in NaCl-treated seedlings as
compared with control revealed (i) quantitative