Full Journal - Journal of Cell and Molecular Biology - Haliç Üniversitesi

al., 2002). In our opinion, the high micronuclei
frequency observed in experimental sample was
induced either by the lagging of whole chromosomes
or the immobility of large acentric fragments. The
formation of acentric fragments resulted from
different chromosome aberrations, and the lagging of
chromosomes was caused by disturbances in the
mitotic spindle or the centromere. We suggest that the
anaphase- and telophase bridges established, as well
as the chromosome fragments resulted from different
types of chromosome aberrations, associated with a
loss of genetic material. Our suggestion corroborated
the data reported by Hoga et al. (1991), who
considered the anaphase bridges as obtained from
structural changes of deficiency- and translocation
type, some of them surviving to late telophase,
indicative of their stability. The increased frequency of
chromosome mutations established by us in sample
No2, agreed with the data reported by Bojadzhiev et
al. (1990), who suggested that lead had embryotoxic
and gonadotoxic effects.
A general conclusion is made that the established
water pollution in the region of the town of
Panagjurishte and its genotoxic effects are a potential
threat to human health. This suggestion supports the
opinion of Ananoshtev et al. (2001) who thought that
the increased cancer rate in the studied region was
associated with the increased contents of lead,
cyanides, arsenic, and cadmium in samples of tap
water and open water sources in the region.
Acknowledgements
This work was financed by the Foundation of
Scientific Investigations of Plovdiv University.
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