Full Journal - Journal of Cell and Molecular Biology - Haliç Üniversitesi

The alterations in protein profiles were recorded in
various cultivars of S. indicum subjected to NaCl
stress. Earlier Pareek et al. (1998) reported changes in
protein profiles in response to NaCl in two cultivars of
rice (Lal Nakanda and Pusa-169). In the present
investigation up-regulation of polypeptides of 81, 61,
65 and 80 kD in the salt-sensitive cultivars RT-125
was recorded. Up- regulation of some specific proteins
like 100 kD and 21 kD in tolerant varieties have been
reported (Pareek et al., 1998; Aarati et al., 2003).
Ashraf and O’Leary (1999) reported decline in certain
proteins in NaCl-sensitive cultivar (cv. potohar) of
wheat as compared to tolerant cultivars LU265 and
Kharchia. Up-regulation of 21.5 kD protein in rice
(Pareek et al., 1998) and 21 kD protein in finger millet
were co-related with NaCl-tolerance (Aarati et al.,
2003). Up-regulation of 100 kD HSP family in NaCland
heat stress-treated seedlings of S. indicum was
confirmed by western blotting. Presence of low level
of this protein even in control seedlings suggests its
constitutional expression.The HSPs perform several
vital functions (protein transport, folding, assembly
and disassembly) during growth and development of
plants but their synthesis increased tremendously upon
heat-stress (Cooper and Ho, 1987; Gething and
Sambrook, 1992).
Acknowledgements
This work was supported by UGC-COSIST & UGC-
DSA under SAP. One of us (AP) thanks J.N.Vyas
University, Jodhpur for providing fellowship out of
UGC-unassigned grant. We are also thankful to Prof.
Anil Grover, Department of Plant Molecular Biology,
South Campus, Delhi University) for providing
facilities to conduct electrophoresis related
experiments, western detection and valuable
suggestios. We acknowledge the help provide by Dr.
S.Rama Rao in formatting the ms.
References
Aarati P, Krishnaprasad BT, Ganeshkumar, Sativa M,
Gopalakrishna R, Ramamohan G. and Udayakumar M.
Expression of an ABA responsive 21 kD protein in
finger millet (Eleusine coracana Gaertn.) under stress
and its relevance in stress tolerance. Plant Sci. 164(1):
25-34, 2003.
Metabolic changes and protein patterns in salinity 37
Ashraf M. Salt tolerance of cotton: some new advances. Crit.
Rev. Plant Sci. 21: 1-30, 2002.
Ashraf M and Harris PJC. Potential biochemical indicators
of salinity tolerance in plants. Plant Sci. 166: 3-16, 2004.
Ashraf M and O’ Leary JW. Changes in soluble proteins in
spring wheat stressed with sodium chloride. Biol Plant.
42: 113-117, 1999.
Ashri A. Genetic resources of Sesame: Present and future
perspective. In: Sesame Biodiversity in Asia,
Conservation, Evaluation and Improvement. Proc. Asia
Regional Workshop of Sesame Evaluation and
Improvement Nagpur and Akola, India. Arora, R.K. and
Riley, K.W. (eds.). IPGRI Office for South Asia, New
Delhi, 25-39, 1993.
Bates LSM, Waldern RP and Teare ID. Rapid determination
of proline for water stress studies. Plant and Soil. 39:
205-207, 1973.
Bolarin MC, Santa-Cruz A, Cayuela E, and Perez-Alfocea F.
Short-term solute changes in leaves and roots of
cultivated and wild tomato seedlings under salinity. J.
Plant Physiol. 147: 463-468, 1995.
Boyer JS. Plant productivity and environment. Science. 218:
444-448, 1982.
Bradford M. A rapid and sensitive method for quantification
of microgram quantities of protein utilizing the principle
of protein dye binding. Anal. Biochem. 72: 248-254,
1976.
Cooper P and Ho TDH. Intracellular localization of heat
shock proteins in maize. Plant Physiol. 84: 1197-1207,
1987.
Delauney AJ and Verma DPS. Proline biosynthesis and
osmoregulation in plants. Plant J. 4: 215-223, 1993.
El-Shintinawy F and El-Shourbagy MN. Alleviation of
changes in protein metabolism in NaCl-stressed wheat
seed by thaimine. Biol Plant. 44: 541-545, 2001.
Flowers TJ, Lachno DR, Flowers TJ and Yeo AR. Some
effects of sodium chloride on cells of rice cultured In
vitro. Plant Science. 39: 205-211, 1985.
Garcia-Reina G, Moreno V and Luque A. Selection for NaCl
tolerance in cell culture of three canary island tomato
land races. I. Recovery of tolerant plantlets from NaCltolerant
cell strains. J. Plant Physiol. 133:1-6, 1988.
Garg AK, Kim JK, Owens TG, Ranwala AP, Do Choi Y,
Kochain LV, and Wu RJ. Trehalose accumulation in rice
plants confers high tolerance levels to different abiotic
stresses. Proc. Natl. Acad. Sci. USA. 99:15898-15903,
2002.
Gehlot HS, Sunita M and Purohit A. Role of specific ions on
growth and metabolic patterns in moth bean (C 3) and
pearl millet (C 4) of Indian desert. Indian J. plant Physiol.
8(2): 145-149, 2003.
Gehlot HS, Upadhyaya A, Davis TD and Sankhla N. Growth
and organogenesis in moth bean callus as affected by
paclobutrazol. Plant Cell Physiol. 30(6): 933-936, 1989.
Gehlot HS, Dinesh R, Choudhary R, Joshi S and Sankhla N.