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Figure 1. Amplification plots for MS COX5B Figure 2. Ct values of COX5B in subjects Cox expression in MS 25 Figure 3. Melting curve for COX 5b gene Figure 4. Standard curve for COX5B gene (determination of index R 2 =0.98).
26 Naeimeeh SAFAVIZAADEH et al. Standarddized results Ct Accordinng to the studiies that were ddone after Real Time PCRR test, the aveerage of standdardized Ct in regard to the state of -actin and GAPDH genes in COX5B among controol and patientts affected with MS are 6.21, 6.71 aand 3.95, 5.446 respectiveely (Figures 55). In additionn, the average of standardizzed Ct in regard to state oof -actin andd GAPDH gennes in COX2 among controls and patiennts affected with MS are 4.86, 4.38 and 4.84, 4.12 resspectively. Figure 5. The Ct COOX5B gene, staandardized wiith ß-actin Table 2. COX55B and COX22 genes expresssion (Ct) on n based of B-aactin referencee gene GGene CCompare COOX5B CCOX2 Taable 3. COX5BB and COX2 ggenes expresssion (Ct) on based b of GAPPDH referencee gene. Gene COX5BB COX22 Patieent Vs Controll 6.71 Patieent vs Controll 4.38 Compare Patiient vs Controol Patiient vs Controol Means (Ct) Std. Er rror mean d 6.21 4.86 Comp parison betweeen the statisttical analysis of CO OX5B t-test annd the resultss showed that there are meaningfful differencees in COX5B gene (P0.05). Onn the other hannd, decreased gene expression iin COX5B wwas observed amon ng MS patiennts rather thhan controls, howe ever, the resullts showed noo meaningful differ rences for the COX2 gene. For equality of va ariances, t-tesst and Levenee’s test were perfo ormed. The results showed that the data relate ed to Ct was not significanntly different COX X5B gene amoong patients and control. Ct comparison c was performed with t-test in patien nts and contrrols. The ressults showed that th he measured t is significantt for COX5B gene (Pvalue=0.0168) and (PPvalue=0.005), while e the data related too Ct has signif ficantly diffferent COX22 gene in patien nts and contrrol. Ct commparison was perfo ormed with t-teest in pateintss and control. The results showwed that meeasured t is signif ficant for COOX2 gene ( (Pvalue=0.117) and (Pvalue=0.124) ( (Table 2, 3) ). Generally, the re esults showedd that actin aand GAPDH genes s are similaar among ccontrol and patien nts. 0.1 19746 0. 1314 0.88 0 Means (Ct) Std.Error S meean df Tva 5.46 3.95 4.12 4.84 1.6 0.89 0.78 1.44 0.69 df Tvalue 664 2.69 00.0168 664 -1.67 Pvalue 0.117 alue Pvalue 64 2.991 0.005 64 -1. 52 0.124
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