final .cdr

NK Sathish et al Visible Spectro Photometric Determination of Darifenacin Hydrobromide in bulk and Pharmaceutical dosage forms
Table 1: Optical characteristics and precision data
Parameter Observation
Absorption maxima (nm) 620 nm
Beer ’ s law limit (µg/ml) 5-25 µg/ml
Correlation coefficient 0.9992
Regression equation (y=mx + c)
Slope (m) 0.0133
Intercept (c) -0.0373
Table 2: Assay of commercial formulations.
Formulation Amount Amount % Assay S.D.* % RSD*
labeled found*
(mg) (mg)/tab
DARILONG 7.5 7.475 99.67 0.421 0.422
7.5 Tablets
DARILONG 15 14.842 98.95 0.298 0.301
15 Tablets
Drug name Amount
label
claimed
(μg/ml)
Darifenacin
HBr (Tablet)
DARILONG
7.5
*Average of six determinations. S.D. is standard deviation, RSD is relative standard deviation.
10
10
10
10
10
10
10
10
10
Drug name Amount
label
claimed
(μg/ml)
10
Darifenacin 10
HBr (Tablet)
10
DARILONG 10
15 10
10
10
10
10
Table 3: Recovery study for tablet formulation (I).
Amount of
standard
added
(μg/ml)
5
5
5
10
10
10
15
15
15
Amount of
standard
added
(μg/ml)
5
5
5
10
10
10
15
15
15
Total
amount
recovered
(μg/ml)
14.768
14.918
14.88
19.932
19.826
19.998
24.745
24.863
24.993
Table 4: Recovery studies for tablet formulation (II).
Total
amount
recovered
(μg/ml)
14.892
14.933
14.964
19.952
19.992
19.996
24.985
24.948
24.995
* Avg. of three determinations, S.D. is standard deviation.
47
Abs
0.3
0.2
0.1
0
400 500 600 700 800
Wavelength(nm)
%
Recovery
*
98.45
99.45
99.2
99.66
99.13
99.99
98.98
99.45
99.97
%
Recovery
*
99.28
99.55
99.76
99.76
99.96
99.98
99.94
99.79
99.98
Mean %
Recovery
(± S.D.)
99.36 ± 0.491
Mean %
Recovery
(± S.D.)
99.78 ± 0.235
RJPS, Apr-Jun, 2011/ Vol 1/ Issue 1