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Lansoprazole (8 mg/kg p.o). ). Group-III and IV were given orally ethanolic extract (250 and 500 mg/kg) of Gossypium herbaceum flowers respectively. They were fasted overnight before the test with free access to water. One hour after the administration of extract and standard drug, absolute ethanol at dose of 1ml/200 g was administered to all the animals. The animals were sacrificed 1h after administration of ethanol and the stomach was removed and opened along the greater curvature. Lesions were examined with the help of hand lens (10X) and sample was sending to further histopathological study. Scoring was done as, 0 = normal stomach, 0.5 = red coloration, 1.0 = spot ulcers, 1.5 = hemorrhagic streaks, 2.0 = ulcer > 3 but < 5 and 3.0 = ulcer > 5. Mean ulcer score for each animal is expressed as ulcer index. The percentage protection was calculated using the following formula; Percentage protection = 100 - Ut / Uc x 100 Where, Ut = ulcer index of treated group. Uc = ulcer index of control group 12 Histopathological evaluation The rats were sacrificed by deep ether anesthesia. The stomach was isolated and preserved in 10% formaldehyde solution for fixation. Then the organ was washed in running tap water for 6-7 hours to remove excess of formaldehyde. Then the organ in capsule was kept in ascending grades of alcohol (70%, 80%, 90% and 95% for 12-15 hours and in absolute alcohol for 6 hours) to remove water from tissue. The clearing of tissue was done by keeping the capsule in Xylene solution for 10-15 minutes. Stomach was then embedded in paraffin wax by boiling for 2 hours and was enclosed in paraffin block. Finally the sections of 4-6 microns were cut on microtome machine. Slides were stained by heamatoxylin eosin staining method and these were examined under the microscope for histopathological changes such as congestion, hemorrhage, necrosis, inflammation, infiltration, erosion and ulcer and photographs were taken. See Figure 3. Statistical analysis Md. Saifuddin Khalid et al Antiulcer activity of Ethanolic extract of Gossypium Harbaceum flowers Results were expressed as mean±SEM, (n=6). Statistical analysis were performed with one way analysis of variance (ANOVA) followed by Dunnett's 't' test P value less than
Md. Saifuddin Khalid et al Antiulcer activity of Ethanolic extract of Gossypium Harbaceum flowers Table 2: Effect of ethanolic extract of Gossypium herbaceum flowers on ulcer index and their % protection in ethanol induced ulcer in rats. Group no Treatment Dose Ulcer index % protection 1 Control 1 ml/200 g, p.o. 7.00±0.632 ---- (absolute ethanol) 2 Lansoprazole 8 mg/kg, p.o 1.66±0.459*** 76.28 3 EEGH 250 mg/kg, p.o 4.83±0.749* 31.00 4 EEGH 500 mg/kg, p.o 2.08±0.436*** 70.28 Values are the mean S.E.M. of 6 rats / treatment, Significant *P < 0.05, **P < 0.01 and ***P < 0.001 compared with Control. Fig.1: Effect of ethanolic extract of Gossypium herbaceum flowers in ethanol induced ulcer in rats. Control Ethanol Standard Lansoprazole (8 mg/kg) 250 mg/kg EEGH 500 mg/kg EEGH 82 RJPS, Apr-Jun, 2011/ Vol 1/ Issue 1
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R G U H S Dr. Divakar Goli Principa
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RGUHS Journal of Pharmaceutical Sci
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Dear Readers, Rajiv Gandhi Universi
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For the determination of surface pH
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S B Shirsand et al Design and Evalu
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The formulation BT containing hydro
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many safer and newer drug delivery
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Compressibility index: This was cal
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colouration indicating the absence
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Heat Flow Endo up (mW) 8 7 6 5 4 3
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cumulative drug release (% CDR) at
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evaluated six replicate injections
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A B S T R A C T RGUHS Journal of Ph
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pharmacokinetics and dynamics, 6 on
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Page 41 and 42: RGUHS Journal of Pharmaceutical Sci
Page 43 and 44: stress. The ISO induced increase in
Page 45 and 46: SMB Asdaqet al Pharmacodynamic inte
Page 47 and 48: CONCLUSION In conclusion, combined
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Page 57 and 58: ACKNOWLEDGEMENT The authors are tha
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