Biochemical and Histopathological Effects of Aflatoxin on ...

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excited by DV light <strong>and</strong> the emitted light is detected by photodetectors <strong>and</strong> recorded on scale set to ppb (parts per billion). Procedure Preparation <strong>of</strong> the micro-column • One end <strong>of</strong> the column was plugged with glass wool. • With the aid <strong>of</strong> a funnel <strong>and</strong> scoop, a layer <strong>of</strong> s<strong>and</strong> about 5 to 7 mm in depth was added. • A layer <strong>of</strong> florisil was added to a depth <strong>of</strong> not more than 5 - 7 mm. • A second layer <strong>of</strong> s<strong>and</strong> was added about 5-7 mm in depth. • A layer <strong>of</strong> silica gel was added about 15 mm in depth. • A layer <strong>of</strong> neutral alumina was added about 15 mm in depth. Development <strong>and</strong> reading <strong>of</strong> the micro-column The prepared column was wetted with chlor<strong>of</strong>orm by lowering bottom <strong>of</strong> the column into vial containing chlor<strong>of</strong>orm. Using a 1 ml syringe, transferred Iml <strong>of</strong> the sample solution from vial into prewetted column <strong>and</strong> allowed to drain in for 2-5 minutes. Added 1 ml <strong>of</strong> chlor<strong>of</strong>orm to column <strong>and</strong> allowed to drain. Prepared simultaneously a similar column with 50 ng (nanogram) <strong>of</strong> st<strong>and</strong>ard <strong>Aflatoxin</strong> Bb which corresponded to 20ppb st<strong>and</strong>ard column. Calibrated the VFM instrument by using both the blank column <strong>and</strong> 20 ppb st<strong>and</strong>ard column so as to read zero <strong>and</strong> 20ppb on the scale respectively. Now placed the sample column in calibrated VFM instrument <strong>and</strong> noted the reading <strong>of</strong> aflatoxin. Next the column was turned around 180 degrees <strong>and</strong> the second reading taken. The final aflatoxin reading was the average <strong>of</strong> the two. 23
Page 1: BIOCHEMICAL AND HISTOPATHOLOGICAL E
Page 6 and 7: CHAPTER 1 Introduction CHAPTER2 Rev
Page 8 and 9: Estimation ofAST E
Page 10 and 11: 1.0 Introduction Aquaculture today
Page 12 and 13: distribution of ma
Page 14 and 15: C H A P T E R Review of</st
Page 16 and 17: 2.3 Structures of
Page 18: animals. As is the case with other
Page 21 and 22: When the dose was increased ten fol
Page 23 and 24: neoplastic cells and</stron
Page 25 and 26: 2.10 b. Carbohydrate and</s
Page 27 and 28: Any discussion in multistage carcin
Page 29 and 30: 3.0 Introduction Fungi grow on pell
Page 31 and 32: imperative to use a method which is
Page 33: MgS04.7H20 FeS0 4.7H20 Distilled wa
Page 37 and 38: All the ingredients were powdered,
Page 39 and 40: methods for primary extraction, the
Page 41 and 42: C H A P T E R 4 Effects</st
Page 43 and 44: 4.1 Source of Reac
Page 45 and 46: 4.3 The Lipid Peroxidation process
Page 47: 4.5 The Antioxidant Defense Mechani
Page 50 and 51: and catalysis. GSH
Page 52 and 53: 4.9.1.c. Estimation of</str
Page 54 and 55: was quickly added and</stro
Page 58: tissues diffe red significantly (p
Page 70: 4.11 Discussion The mechanism <stro
Page 73 and 74: C Histopathology H A P T E R 5
Page 76: 5.3. Results Several changes were o
Page 80 and 81: Aflatoxin Dose 0.3
Page 84 and 85:
In GlIb both proximal convoluted tu
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Cirrhosis (a progressive liver dise
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Both the proximal convoluted tubule
Page 90 and 91:
6.0 Introduction Of the energy reso
Page 92:
oth cytoplasm and
Page 98 and 99:
DNPH reagent was added to both the
Page 100:
Procedure Liver, heart muscle <stro
Page 104 and 105:
8. Glucose oxidase reagent. Added 0
Page 106 and 107:
6.2.12 Estimation of</stron
Page 108 and 109:
chloride precipitating reagent <str
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2) MgCh,( 2M) : Dissolved 40.7 g <s
Page 116:
significantly higher values when co
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35 Cl 30 c. .€. 25 c l: l: Q. '-
Page 124 and 125:
104 by one way ANOVA. LSD values at
Page 126 and 127:
106 Source ofVaria
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-_._--------- ---------- - ----- -
Page 132 and 133:
Source of Variatio
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114 A significant decrease in the l
Page 136 and 137:
7.0 Introduction 115 Advances in mo
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117 present a distinctive sequence
Page 140:
The mixture was dissolved in minimu
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ii. Stacking gel Acrylamide <strong
Page 147:
Stain(500ml) Coomassie Brilliant Bl
Page 150 and 151:
'1.4 _ .. - I " n _ ,, - PLATE·15
Page 153 and 154:
130 is one of the
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132 to see the ladder like DNA frag
Page 157 and 158:
8.0 Studies on the Lysosomal Membra
Page 159 and 160:
equal volume of do
Page 162 and 163:
One way Analysis of</strong
Page 164:
and also between c
Page 167 and 168:
(Shank R C, 1981). Histopat
Page 169 and 170:
distilled water blank. The dilution
Page 172 and 173:
Source of Variatio
Page 174 and 175:
organisms (Haya and</strong
Page 176:
The precipitate 3 thus obtained whi
Page 181 and 182:
and from the contr
Page 183 and 184:
mossambicus, the effects of
Page 185 and 186:
Mobile phase : CH30HlCH3CN/5.0 % CH
Page 187 and 188:
Discussion Treatment P value O.375p
Page 189 and 190:
HDL cholesterol which recorded an i
Page 191 and 192:
different fragments thereby causing
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Ayres J L, Lee DJ, Wales J H<strong
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Clifford J I, Rees K K and<
Page 197 and 198:
George K C (1997). Comparative stud
Page 199 and 200:
Kerr J F R, Wyllie A Hand</
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Mohun AF and Cook
Page 203 and 204:
Plummer DT (1987). An introduction
Page 205 and 206:
Shantha T (1994). Methods for Estim
Page 207 and 208:
Toskulkao C and Gl
Page 209:
Zak B (1957). Amer. 1. Clin. Path.
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