Biochemical and Histopathological Effects of Aflatoxin on ...

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CHAPTER 1 Introduction CHAPTER2 Review <strong>of</strong> Literature CHAPTER3 CONTENTS Extraction And Estimation <strong>of</strong><strong>Aflatoxin</strong> <strong>and</strong> its Incorporation into Fish Feed Introduction Materials <strong>and</strong> Methods Subculture <strong>of</strong>the fungus, Aspergillusflavus Composition <strong>of</strong>Czapek Yeast Extract Agar Extraction <strong>of</strong><strong>Aflatoxin</strong> Extraction Protocol Detection <strong>and</strong> Estimation <strong>of</strong><strong>Aflatoxin</strong> using Flu'i-otoxinmeter " Preparation <strong>of</strong>Fish Feed Results Discussion CHAPTER4 <strong>Effects</strong> <strong>of</strong> <strong>Aflatoxin</strong> on the Lipid Peroxidation Process Introduction Materials <strong>and</strong> Methods Estimation <strong>of</strong>Catalase Estimation <strong>of</strong>Superoxide dismutase 1-4 5-17 18-28 29-59
Estimation <strong>of</strong>Glutathione reductase Estimation <strong>of</strong>Malondialdehyde Estimation <strong>of</strong> Hydroperoxide Estimation <strong>of</strong>Conjugated diene Estimation <strong>of</strong>Glutathione Results Discussion CHAPTER 5 Histopathology Introduction Materials <strong>and</strong> Methods Results Discussion CHAPTER 6 <strong>Aflatoxin</strong> mediated <strong>Biochemical</strong> Changes In Oreochromis mossambicus Introduction Experimental Design Materials <strong>and</strong> Methods Estimation <strong>of</strong> Pyruvate Estimation <strong>of</strong>Urea Estimation <strong>of</strong> Alanine Transaminase Estimation <strong>of</strong> Aspartate Transaminase Estimation <strong>of</strong>Alkaline Phosphatase Estimation <strong>of</strong>Free Amino acids Estimation <strong>of</strong>Protein Estimation <strong>of</strong> Serum protein, Albumin <strong>and</strong> Globulin Estimation <strong>of</strong>Serum LDH Estimation <strong>of</strong>Blood Glucose Estimation <strong>of</strong>Alkaline Phosphatase Estimation <strong>of</strong>Acid Phosphatase Estimation <strong>of</strong>ALT 60-69 70-114
Page 1: BIOCHEMICAL AND HISTOPATHOLOGICAL E
Page 8 and 9: Estimation ofAST E
Page 10 and 11: 1.0 Introduction Aquaculture today
Page 12 and 13: distribution of ma
Page 14 and 15: C H A P T E R Review of</st
Page 16 and 17: 2.3 Structures of
Page 18: animals. As is the case with other
Page 21 and 22: When the dose was increased ten fol
Page 23 and 24: neoplastic cells and</stron
Page 25 and 26: 2.10 b. Carbohydrate and</s
Page 27 and 28: Any discussion in multistage carcin
Page 29 and 30: 3.0 Introduction Fungi grow on pell
Page 31 and 32: imperative to use a method which is
Page 33: MgS04.7H20 FeS0 4.7H20 Distilled wa
Page 36 and 37: 3.3.6 Preparation of</stron
Page 38 and 39: Aflatoxin biosynth
Page 40 and 41: The solubility of
Page 42 and 43: 4.0 Introduction Chemical compounds
Page 44 and 45: Generalized Scheme for Oxidative In
Page 46 and 47: Initiation and pro
Page 49 and 50: (d) Glutathione peroxidase (E.C.l.l
Page 51 and 52: 4.9.l.b Estimation of</stro
Page 53 and 54: Preparation of Tis
Page 55: Procedure Tissues homogenates were
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was 0.559. Concentration 0.375 ppm
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(Madhusudhanan et al, 2000). But in
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5.0. Introduction Structural <stron
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PLATE 8.5 Section of</stron
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The GlIb section of</strong
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All experimental fish appeared norm
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lesion, serving as precursors <stro
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C H A P T E R 6 Aflatoxin</
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Biochemically, afl
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}.2 Materials and
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incubation, 0.2ml of</stron
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times. Waited for 10 minutes till a
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2. Buffer: Sodium carbonate- bicarb
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added to O.Sml of
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Procedure 2g of fl
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diluent solution. Using a commercia
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Parameter Treatment Urea ( J1 moles
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4 3 .... Cl 2 1 o Control 0.375ppm
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300 250 200 "Cm150 E 100 50 o Contr
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Treatment Parameter P value Cholest
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I Source of Variat
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113 type seem to take an active rol
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C H A P T E R 7 Electrophoretic Ana
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116 Among the many biornarkers the
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118 refrigerator for some time to e
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7.1.2b Reagents for electrophoresis
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7.1.2c Preparation of</stro
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3) 10% SDS 4) 6 M Sodium chloride 5
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) Serum Protein (Non SDS Page) 129
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fragments of simil
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C H A P T E R 8 Effect of</
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may result from destabilization <st
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2) Buffer: Citrate buffer (O.05M) <
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Source of Variatio
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Groups Pvalue Control vs 0.375ppm N
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8.1 Studies on the Erythrocyte Memb
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4) To O.lml of the
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8.ID Discussion The results obtaine
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a) Preparation of
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Source of Variatio
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8.3 Studies On Muscle Retention 8.3
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Detection: Waters 440 Absorbance at
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8.3C Results The results are presen
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9.0 Summary The present study was u
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• Aflatoxin resi
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BIBLIOGRAPHY Abedi A Hand</
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Bruenger, A and Gr
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Egmond H P V and W
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Hendricks J D, Wales J H, Sinnhuber
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Lowry 0 H, Rosenbrough N J, Farr A
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New M B (1987). Feed and</s
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Saber N A (1995). Depression <stron
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Soni KB, Rajan A and</stron
Page 208 and 209:
Verma R J, Kolhe A Sand</st
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