Biochemical and Histopathological Effects of Aflatoxin on ...

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LIST OF NOTATIONS AND ABBREVIATIONS IUPAC LDso TLC HPLC ELlSA RIA SOS TEMED ALP ALT AST ANOVA ATPase IU LERA LSD SOD International Union <strong>of</strong> Pure <strong>and</strong> Applied Chemistry Lethal dose causing 50% mortality Thin layer chromatography High pressure liquid chromatography Enzyme Linked ImmunoSorbent Assay Radioimmunoassay Sodium dodecyl sulfate NNNN Tetra methyl ethylenediamine Alkaline phosphatase Alanine transaminase Aspartate transaminase Analysis <strong>of</strong> Variance Adenosine triphosphatase International Unit Lysosomal enzyme release assay Least significant difference Superoxide dismutase.
Page 1: BIOCHEMICAL AND HISTOPATHOLOGICAL E
Page 7 and 8: Estimation ofGluta
Page 9 and 10: C H A P T E R Introduction 1
Page 11 and 12: are probably the causes for the pre
Page 13 and 14: 1. To document the biochemical chan
Page 15 and 16: 2.0. Review of lit
Page 17 and 18: 2.4. Biotransformation of</
Page 20 and 21: Enzyme profile <st
Page 22 and 23: in gross hepatoma in 44 of<
Page 24 and 25: However, only aflatoxinls, is consi
Page 26 and 27: ._ the former salmonid is clearly m
Page 28 and 29: C H A P T E R 3 Extraction
Page 30 and 31: pigment content require a different
Page 32 and 33: PUlE·1 ASPERGILLUS FLAVUS CULTURED
Page 35 and 36: excited by DV light and</st
Page 37 and 38: All the ingredients were powdered,
Page 39 and 40: methods for primary extraction, the
Page 41 and 42: C H A P T E R 4 Effects</st
Page 43 and 44: 4.1 Source of Reac
Page 45 and 46: 4.3 The Lipid Peroxidation process
Page 47: 4.5 The Antioxidant Defense Mechani
Page 50 and 51: and catalysis. GSH
Page 52 and 53:
4.9.1.c. Estimation of</str
Page 54 and 55:
was quickly added and</stro
Page 58:
tissues diffe red significantly (p
Page 70:
4.11 Discussion The mechanism <stro
Page 73 and 74:
C Histopathology H A P T E R 5
Page 76:
5.3. Results Several changes were o
Page 80 and 81:
Aflatoxin Dose 0.3
Page 84 and 85:
In GlIb both proximal convoluted tu
Page 86 and 87:
Cirrhosis (a progressive liver dise
Page 88 and 89:
Both the proximal convoluted tubule
Page 90 and 91:
6.0 Introduction Of the energy reso
Page 92:
oth cytoplasm and
Page 98 and 99:
DNPH reagent was added to both the
Page 100:
Procedure Liver, heart muscle <stro
Page 104 and 105:
8. Glucose oxidase reagent. Added 0
Page 106 and 107:
6.2.12 Estimation of</stron
Page 108 and 109:
chloride precipitating reagent <str
Page 110 and 111:
2) MgCh,( 2M) : Dissolved 40.7 g <s
Page 116:
significantly higher values when co
Page 121 and 122:
35 Cl 30 c. .€. 25 c l: l: Q. '-
Page 124 and 125:
104 by one way ANOVA. LSD values at
Page 126 and 127:
106 Source ofVaria
Page 128:
-_._--------- ---------- - ----- -
Page 132 and 133:
Source of Variatio
Page 134 and 135:
114 A significant decrease in the l
Page 136 and 137:
7.0 Introduction 115 Advances in mo
Page 138 and 139:
117 present a distinctive sequence
Page 140:
The mixture was dissolved in minimu
Page 144 and 145:
ii. Stacking gel Acrylamide <strong
Page 147:
Stain(500ml) Coomassie Brilliant Bl
Page 150 and 151:
'1.4 _ .. - I " n _ ,, - PLATE·15
Page 153 and 154:
130 is one of the
Page 155 and 156:
132 to see the ladder like DNA frag
Page 157 and 158:
8.0 Studies on the Lysosomal Membra
Page 159 and 160:
equal volume of do
Page 162 and 163:
One way Analysis of</strong
Page 164:
and also between c
Page 167 and 168:
(Shank R C, 1981). Histopat
Page 169 and 170:
distilled water blank. The dilution
Page 172 and 173:
Source of Variatio
Page 174 and 175:
organisms (Haya and</strong
Page 176:
The precipitate 3 thus obtained whi
Page 181 and 182:
and from the contr
Page 183 and 184:
mossambicus, the effects of
Page 185 and 186:
Mobile phase : CH30HlCH3CN/5.0 % CH
Page 187 and 188:
Discussion Treatment P value O.375p
Page 189 and 190:
HDL cholesterol which recorded an i
Page 191 and 192:
different fragments thereby causing
Page 193 and 194:
Ayres J L, Lee DJ, Wales J H<strong
Page 195 and 196:
Clifford J I, Rees K K and<
Page 197 and 198:
George K C (1997). Comparative stud
Page 199 and 200:
Kerr J F R, Wyllie A Hand</
Page 201 and 202:
Mohun AF and Cook
Page 203 and 204:
Plummer DT (1987). An introduction
Page 205 and 206:
Shantha T (1994). Methods for Estim
Page 207 and 208:
Toskulkao C and Gl
Page 209:
Zak B (1957). Amer. 1. Clin. Path.
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