Toxicity measurements in concentrated water samples - Rivm

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Appendix I. Protocols Inclusion of all protocols in the printed version would mean a large number of pages and a lot of paper. Therefore, the protocols will be included only in the on-line edition of the report, which can be found at www.rivm.nl/bibliotheek/rapporten/607013010.html I.1 Sampling I.2 XAD extraction I.3 Microtox ® assay I.4 Algae: PAM-test I.5 Algae: Fluorescence test (growth) I.6 Daphnia IQ test I.7 Daphnia immibility test I.8 Thamnotoxkit F I.9 Rotoxkit F I.10 Chydorid test RIVM Report 607013010 107
108 RIVM Report 607013010
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Report 607013010/2009 A.M. Durand e
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© RIVM 2009 Parts of this publicat
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4 Toxicity testing 51 4.1 Choice of
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• endpoints mortality, growth inh
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De biologische methoden zijn gesele
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environmental samples respond to th
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For less than 4 test species, the s
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is sufficient. The system used shou
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water sample with the XAD (Figure 3
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3.3.1 Extraction from the water sam
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- the affinity of the hydrophilic p
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3.3.2 Elution from XAD Solvent sele
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Water concentrates need to be analy
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factor. Substances with a vapour pr
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a % of nominal initial concentratio
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% of nominal initial concentration
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After KD-distillation the recovery
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Too much water in an acetone eluate
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The experiments with other chemical
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3.6 Conclusions The method seems su
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Table 4-1 gives an overview of the
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Figure 4-2. Pseudokirchneriella sub
Page 57 and 58: Figure 4-3. Daphnia magna, adult 2
Page 59 and 60: Figure 4-7. Chydorus sphaericus, ad
Page 61 and 62: tests in the range of 25-50 %. In c
Page 63 and 64: In the chemical risk assessment an
Page 65 and 66: this purpose, a concentration accor
Page 67 and 68: The calculated pT value is very use
Page 69 and 70: effects on these important groups a
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Page 75 and 76: possible. In case an analytical met
Page 77 and 78: Table6-4. Repeatability of the tota
Page 79 and 80: during the extraction procedure, in
Page 81 and 82: 0 -14 -12 -10 -8 -6 -4 -2 0 standar
Page 85 and 86: Lobith and Eijsden, where these riv
Page 87 and 88: 20 16-20 11-15 6-10 2-5 Nieuwe Wate
Page 89 and 90: In a second sampling campaign the i
Page 91 and 92: Figure 7-6. Maximum effect values s
Page 93 and 94: Preliminary experiments suggest tha
Page 95 and 96: In planning monitoring activities u
Page 97 and 98: their natural environment. However,
Page 103 and 104: Dekker T, Greve GD, Ter Laak TL, Bo
Page 105 and 106: Peeters ETHM (2001) Benthic macroin
Page 107: Willemsen A, Vaal MA, De Zwart D (1
Page 111 and 112: Substance type Substance CAS nr mol
Page 113 and 114: approach is that it ignores the pre
Page 115: RIVM National Institute for Public
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Toxicity measurements in concentrated water samples - Rivm

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