production of animal proteins by cell systems - New Harvest

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equired do not much extend beyond those for single-celled (eukaryotic) microorganisms. This is largely due to the considerable shear-stress resistance of these cells. Besides shear-stress resistance, two other major factors in the scale-up of the growth of animal cells is their dependence on (i) specific growth factors and (ii) a solid support. Literature 1. Giesecke, D., S. Gaebler, and W. Tiemeyer, Purine Availability and Metabolism in Dogs Fed Single-Cell Protein or Rna. Journal of Nutrition, 1982. 112(10): p. 1822-1826. 2. Colla, L.M., et al., Production of biomass and nutraceutical compounds by Spirulina platensis under different temperature and nitrogen regimes. Bioresour Technol, 2007. 98(7): p. 1489- 93. 3. Sikkeland, L.I., et al., Complement activation and cytokine response by BioProtein, a bacterial single cell protein. Clin Exp Immunol, 2007. 148(1): p. 146-52. 4. Berg, J.J., Tymoczko, J.L., Stryer, L., Biochemistry. Sixth Edition ed. 2006, New York: W.H. Freeman and Company. 5. Woodham, A.A. and E.M. Clarke, Nutritive value of mixed proteins. 2. As determined by net protein utilization and protein efficiency ratio tests. Br J Nutr, 1977. 37(3): p. 309-19. 6. Moon N.J., H.E.G., Glatz B.A., Conversion of cheese whey and whey permeate to oil and single-cell protein. Dairy Science, 1978. 61: p. 1537-1547. 7. Evenblij, M., Veroudering en DNA schade. Mediator, 2009. 20(3): p. 8-9. 8. Garinis, G.A., et al., Persistent transcription-blocking DNA lesions trigger somatic growth attenuation associated with longevity. Nature Cell Biology, 2009. 11(5): p. 604-U370. 9. Gudbrandsen, O.A., et al., Dietary single cell protein reduces fatty liver in obese Zucker rats. Br J Nutr, 2008. 100(4): p. 776-85. 10. Chan, L.C., P.F. Greenfield, and S. Reid, Optimising fed-batch production of recombinant proteins using the baculovirus expression vector system. Biotechnol Bioeng, 1998. 59(2): p. 178-88. *essential amino acids: Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine *semi-essential (in young children): Tyrosine, Cysteïne, Histidine, Arginine 54
List of speakers at the first in vitro meat symposium This symposium was held at the Norwegian Food Research Institute (Matforsk), Aas, Norway, hosted by the Norwegian University of Life Sciences (UMB) and the Norwegian Food Research Institute (Matforsk), April 9-11 2008. The authors of this report are not included. Expertise: Founder of New Harvest (www.new-harvest.org) Jason Matheny, New Harvest & Johns Hopkins University, Baltimore, MD, USA Expertise: Myogenic cells/Biotechnology Prof. Dr. Tor Erling Lea Prof. Dr. Stig W. Omholt Norwegian University of Life Sciences, Aas, Norway Expertise: Market motivation Prof. Dr. Elizabeth L. DeCoux, Florida Coastal School of Law, Jacksonville, FL, USA Expertise: Large scale fractionation of cell extracts Dr. Arild Johannessen, International Research Institute of Stavanger, Stavanger, Norway Expertise: Synthetic serum-free media for cell cultures Dr. Kjell Bertheussen, University of Tromsø, Tromsø, Norway Expertise: Bioreactors for tissue engineering Prof. Dr. Jose Teixeira Dr. Manuela Gomes, University of Minho, Braga, Portugal Expertise: Muscle tissue engineering Robert G. Dennis, UNC NCSU, University of North Carolina, Chapel Hill, NC, USA Expertise: Experimental and theoretical biophysics of muscle tissue Dr. Poul Nielsen, Department of Engineering Science, The University of Auckland, Auckland, New Zealand Expertise: Large and complex bio-engineering plants Dr. Gunnar Kleppe, Norwegian Bioindustry Association, Oslo, Norway 55
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FACULTEIT DIERGENEESKUNDE / FACULTY
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Production of animal proteins by ce
Page 5 and 6: Boxes: 08 Need for protein sources
Page 7 and 8: Introduction The use of livestock f
Page 9 and 10: Cultured meat: a short history The
Page 11 and 12: Cell culture Prokaryotic cells. Res
Page 13 and 14: In warm-blooded animals, such as ma
Page 15 and 16: encode for animal growth factors ca
Page 17 and 18: Skeletal muscle from stem cells Alt
Page 19 and 20: limited number of cell types. This
Page 21 and 22: on flexible membranes. Mounting the
Page 23 and 24: Experts’ opinions (3): Technologi
Page 25 and 26: Best of both worlds In their paper
Page 27 and 28: 4. Tissue engineering of muscle fib
Page 29 and 30: products of cultured algae. Technol
Page 31 and 32: Current developments in cultured me
Page 33 and 34: The proliferative and differentiati
Page 35 and 36: Scientific publications from the 'I
Page 37 and 38: production of chicken meat equals a
Page 39 and 40: Experts’ opinions (7): The most i
Page 41 and 42: Experts’ opinions (8): The role o
Page 44 and 45: Experts’ opinions (9): Potential
Page 47 and 48: Summary The global population is es
Page 49 and 50: knowledge is lacking with respect t
Page 51 and 52: elang om gedegen wetenschappelijk o
Page 53 and 54: 31. Yu, J. et al. Induced pluripote
Page 55: Alternative (animal) cell systems f
Page 59 and 60: Publications about in vitro meat li
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