3 - UPLC_Work_Practical_Considerations

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Running U-HPLC: Practical Considerations Automated Method Development LAN LAC/E Card Network 4-Relay Panel Shula Levin, Waters Israel Solvent Valve Assembly ACQUITY UPLC H-Class Target is end 1Q10 Auto•Blend: Constitute the Mobile Phase on the Fly. Water Acetonitrile Alcohol Concentrated Modifier 14
Running U-HPLC: Practical Considerations 95% Water 0% Isopropanol 100% Water 0% Acetonitrile 0.05% TFA AU AU AU 0.15 0.10 0.05 0.00 0.25 0.20 0.15 0.10 0.20 0.15 0.10 0.05 Auto•Blend Gradient 0% Acetonitrile Water 5% 1%TFA 45% 0% Isopropanol 50% Acetonitrile 5% 1%TFA Peptide Map Development Varying TFA Concentration - %D Shula Levin, Waters Israel 50% Water 50% Acetonitrile 0.05% TFA 0.025% TFA – 2.5% D 0.05% TFA – 5% D 0.1% TFA – 10% D 27.00 27.50 28.00 28.50 29.00 29.50 30.00 30.50 31.00 31.50 32.00 32.50 33.00 33.50 34.00 34.50 35.00 35.50 36.00 36.50 37.00 Minutes 15
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