3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
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Running U-HPLC: <strong>Practical</strong> <strong>Considerations</strong><br />
Full Loop Injection<br />
Example of a 20 µL injection with a 4X overfill<br />
20 µL Sample<br />
Weak Wash<br />
Solvent<br />
Air Gap<br />
Sample<br />
Sample<br />
Weak Wash<br />
Solvent<br />
Air Gap<br />
30 µL<br />
sample<br />
volume<br />
Sample Loop<br />
Volume Injected<br />
Overfill Factor 1 to 4<br />
30 µL<br />
sample<br />
volume<br />
Full Loop Injection<br />
Step 1 – Aspirate sample and air gap<br />
Shula Levin, Waters Israel<br />
28
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