3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
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Running U-HPLC: <strong>Practical</strong> <strong>Considerations</strong><br />
• Question:<br />
– 1) Should I use air gap?<br />
• Yes or No?<br />
• Which one?<br />
Air Gap<br />
– Pre air, post air or both?<br />
– 2) What volume?<br />
• Question:<br />
– Should I use?<br />
• Full loop<br />
• Partial loop injection<br />
Pre and Post Air Gap Influence: Example<br />
4 4 1 140787 132577 96240 57406 136252 95577 45260 16142<br />
0 0 1 90362 85040 62005 42553 92886 64077 30046 12199<br />
Diffusion effect 55.80% 55.90% 55.21% 34.90% 46.69% 49.16% 50.64% 32.32%<br />
4 4 1 140787 132577 96240 57406 136252 95577 45260 16142<br />
0 4 1 89291 84372 63720 40764 85074 61209 29754 11578<br />
Diffusion effect 57.67% 57.13% 51.04% 40.83% 60.16% 56.15% 52.11% 39.42%<br />
4 4 1 140787 132577 96240 57406 136252 95577 45260 16142<br />
4 0 1 120864 113953 81763 52170 122771 85128 39046 14816<br />
Diffusion effect<br />
16.48% 16.34% 17.71% 10.04% 10.98% 12.27% 15.91% 8.95%<br />
Shula Levin, Waters Israel<br />
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