3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
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Running U-HPLC: <strong>Practical</strong> <strong>Considerations</strong><br />
The practice of <strong>UPLC</strong> operation is the same whether it is a Binary or a<br />
Quaternary System<br />
Solvent Management<br />
ACQUITY <strong>UPLC</strong> ACQUITY <strong>UPLC</strong> H-Class<br />
Solvent Manager Design Binary - 2 solvents Quaternary - 4 solvents<br />
Total Number of Solvents Solvents A1 or A2 and B1 or B2<br />
Solvents A, B, C, D<br />
(Solvents D 1-6 via SSV option)<br />
Type of Solvent Mixing High pressure mixing Low pressure mixing<br />
System Volume < 100uL standard configuration < 400uL standard configuration<br />
Column I.D. 1.0 mm to 4.6 mm 2.1 mm to 4.6 mm<br />
Pressure/Flow Performance<br />
15,000 psi up to 1mL/min<br />
9,000 psi up to 2mL/min<br />
Flow Rate Range 0.01 to 2mL/min<br />
Shula Levin, Waters Israel<br />
2
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