3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
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Running U-HPLC: <strong>Practical</strong> <strong>Considerations</strong><br />
Injector design examples<br />
Flow-Through variable injector design<br />
0.040<br />
0.030<br />
0.020<br />
AU<br />
0.010<br />
0.000<br />
-0.010<br />
loop<br />
Alliance 2695 and H-Class <strong>UPLC</strong><br />
Mounting of the loop<br />
MUST BE PROPER!!<br />
5.605.705.805.906.006.106.206.306.40<br />
Minutes<br />
Typical problem with bad peek<br />
connections<br />
First blank 0.1 %<br />
Second blank 0.01 %.<br />
.<br />
ACQUITY fixed loop injector design<br />
(Rheodyne)<br />
Shula Levin, Waters Israel<br />
loop<br />
With proper Peek<br />
connections<br />
First blank
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