3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
3 - UPLC_Work_Practical_Considerations
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Running U-HPLC: <strong>Practical</strong> <strong>Considerations</strong><br />
General Operating<br />
Recommendations<br />
• Use only Pyrex (Borosilicate 3.3) bottles. DURAN bottles<br />
dissolve at high pH (>9).<br />
• Use highest quality solvents, water, buffers and additives.<br />
• Flush buffers out of system with water after use (use 10-<br />
20% organic in water for storage).<br />
• Keep all 4 solvent lines primed (use 10-20% organic in<br />
water for unused lines).<br />
• Keep seal wash primed.<br />
• Re-prime solvent lines before starting.<br />
• Use 100 µL mixer for TFA/ACN gradients at low<br />
wavelengths.<br />
CORNING PYREX ® Type 1, Class A, Borosilicate Bottles<br />
SCHOTT DURAN ® Borosilicate Glass 3.3 Bottles<br />
Also: if Biolab solvents: Use ULC/MS grade only!<br />
Shula Levin, Waters Israel<br />
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