3 - UPLC_Work_Practical_Considerations

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Running U-HPLC: Practical Considerations Sample Manager –FTN Sample Loading Sample Manager –FTN Sample Injection Shula Levin, Waters Israel 18
Running U-HPLC: Practical Considerations Injection Performance 2 Orders of Magnitude No re-configuration necessary across the entire UPLC injection volume range Area Area Area 300000 200000 100000 10.0µL @ 2µg/mL 1.0µL @ 20µg/mL 0.1µL @ 200µg/mL Same peak area Linearity Test: Injection Volumes 0 Calibration Plot 0.00 2.00 4.00 6.00 8.00 10.00 12.00 Injection Volume uL Name: acetanilide; Fit Type: Linear (1st Order); Equation Y = 2.66e+004 X - 8.70e+002; R^2 0.999997 50000.0 40000.0 30000.0 20000.0 10000.0 0.0 Calibration Plot 0.00 2.00 4.00 6.00 8.00 10.00 12.00 Injection Volume uL Name: propiophenone; Fit Type: Linear (1st Order); Equation Y = 3.47e+003 X - 1.09e+002; R^2 0.999996 250000 200000 150000 100000 50000 0 Calibration Plot 0.00 2.00 4.00 6.00 8.00 10.00 12.00 Injection Volume uL Name: benzophenone; Fit Type: Linear (1st Order); Equation Y = 1.85e+004 X - 5.74e+002; R^2 0.999993 Area Area Area 100000.0 80000.0 60000.0 40000.0 20000.0 0.0 Calibration Plot 0.00 5.00 10.00 Injection Volume uL Name: acetophenone; Fit Type: Linear (1st Order); Equation Y = 7.40e+003 X - 2.47e+002; R^2 0.999998 250000 200000 150000 100000 50000 0 Calibration Plot 0.00 2.00 4.00 6.00 8.00 10.00 12.00 Injection Volume uL Name: butylparaben; Fit Type: Linear (1st Order); Equation Y = 1.87e+004 X - 6.19e+002; R^2 0.999991 20000.0 15000.0 10000.0 5000.0 0.0 Calibration Plot 0.00 2.00 4.00 6.00 8.00 10.00 12.00 Injection Volume uL Name: valerophenone; Fit Type: Linear (1st Order); Equation Y = 1.44e+003 X - 1.29e+000; R^2 0.999964 Shula Levin, Waters Israel 19
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3 - UPLC_Work_Practical_Considerations

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