PHYS01200704032 Debes Ray - Homi Bhabha National Institute

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Chapter 7: Interaction of Gold Nanoparticle with Proteins Figure 7.13 shows SANS data of gold nanoparticles (0.01 wt% P85 + 0.2 wt% HAuCl 4 .3H 2 O + 0.2 wt% Na 3 Ct) with varying concentration of BSA protein. All these nanoparticle-protein conjugate systems are found to be stable. SANS data of the conjugates show the almost similar scattering patterns in the low-Q region and difference are observed at the larger Q values. The SANS data from corresponding pure protein solutions are given in the inset of Figure 7.13. The data at higher protein concentrations show correlation peak of structure factor an indication of interacting protein in the solution. The data of nanoparticleprotein conjugate suggest that the adsorbed protein on the nanoparticles surface does not vary much with the increase in the protein concentration. The build-up of scattering in the higher Q region with increase in protein concentration is as a result of increase in the free protein (not adsorbed on the nanoparticle) concentration. 7.4. Conclusions The optimization of high yield synthesis of gold nanoparticle for probing their interaction with proteins has been examined. The stable and high-yield gold nanoparticles have been synthesized at very low block copolymer concentration (decrease of 3 orders) to minimize any direct interaction of proteins with the block copolymer. The faster formation rate of gold nanoparticles is found with higher value of block copolymer concentration in these systems. The stability and yield of nanoparticle remain same irrespective of the large decrease in block copolymer concentration. The nanoparticle structure is also found identical irrespective of block copolymer concentration. The interaction of these gold nanoparticles with two model proteins [lysozyme and bovine serum albumin (BSA)] has been examined. It has been found that gold nanoparticles form stable solutions over a wide concentration range of BSA whereas phase separate even with small amount of lysozyme protein at physiological conditions. These results can be explained on the basis of that citrate ions are adsorbed on the gold nanoparticles make it negative, as a result their complex with positively charged lysozyme phase separates whereas it remains stable with similarly charged BSA. The 170
Chapter 7: Interaction of Gold Nanoparticle with Proteins complexes of gold nanoparticles with BSA have been studied using UV-visible spectroscopy, zeta potential and SANS. The red shift in the SPR peak and the build-up of scattering intensity in the low-Q region of SANS data confirms the adsorption of protein on nanoparticles. The increase in zeta potential of the gold nanoparticles in the presence of BSA also support to the conjugation of protein with nanoparticles. The adsorption of protein on the gold nanoparticles has been modeled in SANS by a core-shell structure consisting of adsorbed protein forming a shell around the gold nanoparticle. 171
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SYNTHESIS AND CHARACTERIZATION OF B
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STATEMENT BY AUTHOR This dissertati
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DECLARATION I, hereby declare that
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Acknowledgements I would like to ex
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2. BLOCK COPOLYMER-MEDIATED SYNTHES
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5. CORRELATING BLOCK COPOLYMER SELF
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SYNOPSIS OF THE THESIS TO BE SUBMIT
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synthesis for its dependence on gol
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interactions between the templating
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utilized in the formation of nanopa
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nanoparticle-protein conjugate conf
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LIST OF FIGURES Figure 1.1. The len
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Figure 4.2. UV-visible absorption s
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Figure 5.9. (a) Absorbance (Abs max
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Figure 7.5. Integrated absorbance o
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Chapter 1 Synthesis, Characterizati
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Chapter 1: Synthesis, Characterizat
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Chapter 2 Block Copolymer-mediated
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Chapter 2: Block Copolymer-mediated
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Time-dependent Absorbance Chapter 2
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Chapter 3: Multi-Technique Approach
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d/d (Q) (cm -1 ) S(Q) P(Q) Chapter
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d/d (cm -1 ) P(Q) Chapter 3: Multi-
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Chapter 4 Optimization of the Block
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Chapter 4: Optimization of the Bloc
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SPR Peak Absorbance Absorbance Chap
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Absorbance Integrated Absorbance /
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Absorbance Chapter 4: Optimization
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SPR peak absorbance Chapter 4: Opti
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d/d (cm -1 ) Chapter 4: Optimizatio
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g I ()-1 Integrated Scattering Inte
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Relative number (%) Chapter 4: Opti
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Chapter 5 Correlating Block Copolym
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Chapter 5: Correlating Block Copoly
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d/d (cm -1 ) Chapter 5: Correlating
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Absorbance Absorbance Chapter 5: Co
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PHYS01200704032 Debes Ray - Homi Bhabha National Institute

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