- Page 1: MICRO-STRUCTURE ANALYSIS OF PLANT T
- Page 5 and 6: PREFACE The subject matter of resea
- Page 7 and 8: PREFACE ...........................
- Page 9 and 10: 1. THE GOAL OF IMAGE ANALYSIS OF PL
- Page 11 and 12: within an object, caused e.g. by a
- Page 13 and 14: 1.4. Analysis of plant tissue struc
- Page 15 and 16: 2. MICROSCOPES AND SAMPLE PREPARATI
- Page 17 and 18: focusing mechanism permitting the a
- Page 19 and 20: 3 5 9 6 7 8 4 2 1 Fig. 2.4. The str
- Page 21 and 22: 2.1.3. Mechanical elements Microsco
- Page 23 and 24: 2.1.4. Image generation in optical
- Page 25 and 26: 2.1.5. Resolution of the microscope
- Page 27 and 28: a) 1 b) Relative refractive index 3
- Page 29 and 30: 2.1.7. Microscopy studies Microscop
- Page 31 and 32: One should take care, however, that
- Page 33 and 34: may be difficult and the objects fi
- Page 35 and 36: Object slicing and application of s
- Page 37 and 38: them to straighten out without dama
- Page 39 and 40: Fig. 2.15. Reconstruction of the tr
- Page 41 and 42: Fig. 2.16. Images of the same part
- Page 43 and 44: Fig. 2.17. Images of the structure
- Page 45 and 46: 2.2. Confocal Microscopy Artur Zdun
- Page 47 and 48: Fig. 2.19. Potato tuber tissue. The
- Page 49 and 50: Fig. 2.21. Confocal Microscope oper
- Page 51 and 52: Fig. 2.22. Potato tuber tissue. Ima
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in spite of the different original
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estimated that for most animal tiss
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However, because of the broadening
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2.3. Electron Microscopy Andrzej Kr
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Due to the high vacuum inside the m
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Fig. 2.38 presents an image of aggr
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Fig. 2.38. Aggregation of starch gr
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a) b) c) d) Fig. 2.40. Schematics o
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Fig. 2.42. Schematic of ESEM detect
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Table 2.1. TECHNOLOGY LIMITING FACT
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3. DIGITAL IMAGES AND THE POSSIBILI
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Fig. 3.1. Image processing and anal
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Table 3.1. Comparison of selected f
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300 dpi, which corresponds to the p
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If we consider a recording element
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Fig. 3.4. Comparison of a circular
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Fig. 3.6. Changing depth of digital
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Fig. 3.7. Change in the appearance
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Fig. 3.8. Comparison of an image in
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Fig. 3.10. Determination of the num
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Maximum width This measurement can
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Fig. 3.11. Counting particles visib
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Let us analyse the distribution of
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An example of the application of co
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Fig. 3.16. The process of binarizat
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Image analysis software packages fr
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image is marked with the letter a).
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The effect of operation of these fi
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And another example of a filter: -1
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3.8. Mathematical morphology Morpho
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and the whole operation is repeated
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The simplest problem is presented i
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Fig. 3.29 illustrates the phenomeno
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Fig. 3.31. Model of RGB. Descriptio
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And substitution is an operation th
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increase decrease 255 contrast + 40
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Fig. 3.37. Application of normaliza
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4.1.2. Fixation of tissue structure
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The format most frequently used for
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Fig. 4.4. Histogram of grey levels
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orderline of two or three cells. Th
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Shape can also be described by mean
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Fig. 4.11. Image of the parenchyma
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4.2. Analysis of images obtained wi
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lens. However, the realization of t
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is now extremely easy, as all the w
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Fig. 4.16. Combination of erosion,
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Fig. 4.20. Division of binary image
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Fig. 4.24. Image labelling (AphImgC
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Fig. 4.28. Repeat watershed detecti
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as 60%), then the source of error i
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in natural state maintaining high s
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Therefore, the tasks of the compute
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Fig. 4.36. Result of Watershed oper
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and 5617 after correction. For carr
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Fig. 4.38. Histograms of cell area,
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should be analyzed separately. An e
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) Fig. 4.42. Result of reconstructi