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2008 Pittcon Final Program - Pittcon Web Archives

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3:45 (2990-7) Single-Cell Electroporation Using 2 Single-Cell<br />

Electroporation Using 2 µm Tip Capillaries with Improved Distance<br />

Control, Temperature Control and Cell Synchronization MANYAN WANG,<br />

University of Pittsburgh, Aparna Agarwal, Bradley A Lambie, Owe Orwar,<br />

Stephen G <strong>Web</strong>er<br />

4:05 (2990-8) Characterization of Giant Mitochondria in Rat<br />

Myoblasts and Skeletal Muscle Tissue MARIAN NAVRATIL, University of<br />

Minnesota, Vratislav Kostal, LaDora Thompson, Edgar A Arriaga<br />

ORAL SESSION Session 3000<br />

Liquid Chromatography/Mass Spectroscopy: Biological<br />

Applications II<br />

Thursday Afternoon, Room 260<br />

Eduard Rogatsky, Albert Einstein College of Medicine, Presiding<br />

1:30 (3000-1) Biological Agent Classification and Identification<br />

Using Mass Spectrometry-Based Bioinformatics Proteomics Approach<br />

RABIH E JABBOUR, SAIC, Jacek P Dworzanski, Samir Deshpande, Melissa<br />

Pryce, Jody L Mays, Stacey M Broomall, Kevin T Kearns, Charles H Wick, Alan<br />

W Zulich<br />

1:50 (3000-2) Validation of a High Performance Liquid<br />

Chromatography-Particle Beam Mass Spectrometry (HPLC-PB/MS) with<br />

Electron Impact (EI) and Glow Discharge (GD) Ionization Sources for<br />

Botanical Extracts JOAUDIMIR CASTRO, Clemson University, R Kenneth<br />

Marcus, M.V. Balarama Krishna<br />

2:10 (3000-3) Increasing the Speed of LC/MS/MS Analysis in<br />

Pharmacokinetic Study Using Microflow HPLC and Sub-Three µm Particles<br />

HUNG-YUAN CHENG, Eksigent Technologies<br />

2:30 (3000-4) Ion Suppression as a Function of Mobile Phase pH in<br />

the Analysis of Polar Basic Drug Compounds in Samples of Biological<br />

Origin by ES+ LC/MS/MS TIVADAR FARKAS, Phenomenex, Inc., Liming<br />

Peng<br />

2:50 Recess<br />

3:05 (3000-5) Metabolic Profiling of Phospholipids in Rat Plasma<br />

Utilizing Ultra Pressure Liquid Chromatography and TOF Mass<br />

Spectrometry PAUL D RAINVILLE, Waters Corporation, Robert S Plumb<br />

3:25 (3000-6) Capillary LC-MS for High Sensitivity Metabolomic<br />

Analysis of Islets of Langerhans QIHUI NI, University of Michigan, Robert<br />

Kennedy<br />

3:45 (3000-7) Determination of Glutathione Conjugates of 4-<br />

Hydroxy-2(E)-Nonenal as Indicators of Transport MOLLY M WARNKE,<br />

University of Texas, Arlington, Sanjay Awasthi, Daniel W Armstrong<br />

4:05 (3000-8) Non-Digest Liberation of Biomarkers from Plasma:<br />

A Novel Two-Stage Ultrafiltration Approach EDUARD ROGATSKY, Albert<br />

Einstein College of Medicine, Daniel T Stein<br />

ORAL SESSION Session 3010<br />

Liquid Chromatography: Pharmaceutical Applications<br />

Thursday Afternoon, Room 261<br />

Peter Rahn, Phenomenex, Presiding<br />

3:25 (3010-6) Parallel Multiple Channel Microflow Liquid<br />

Chromatographic Method Development for Complex Separations: An<br />

Immerging Instrumental Technique DAVID HUGHES, Chromatographic<br />

Excellence, LLC, Laurie Allan, Jason Starkey, Don W Arnold<br />

3:45 (3010-7) Evaluation of New 3 Micron HILIC Column for<br />

Hydrophilic Compounds HIROSHI TOMIZAWA, Tosoh Corporation, Emi<br />

Sakima, Hiroyuki Yamasaki, Toshiaki Nishi, Hiroyuki Moriyama, Yoichi Yasuda,<br />

Cara Tomasek<br />

4:05 (3010-8) Column Diameter’s Role in the Performance of Small<br />

Scale Preparative Columns PETER RAHN, Phenomenex, Gareth Friedlander,<br />

Graham Osborn<br />

ORAL SESSION Session 3020<br />

Liquid Chromatography: Pharmaceutical Applications: Chiral<br />

Separations (Half Session)<br />

Thursday Afternoon, Room 271<br />

Perry G Wang, Teleflex, Presiding<br />

3:05 (3020-1) Chiral HPLC/SFC Prep Purification: Robust<br />

Collection of Enantiomer Peaks Using the +/- Derivative of an Advanced<br />

Laser Polarimeter Signal GARY W YANIK, PDR-Chiral<br />

3:25 (3020-2) A Chiral Piece of the Pie: Optimizing Chiral<br />

Separations through Selective Sample Slicing and Column Switching<br />

MARK E CRAWFORD, Gilson Inc., Joan Marie Stevens<br />

3:45 (3020-3) Optimized Chiral Separations and Enantiomeric<br />

Excess Determined via an Automated Parallel Chromatographic Scouting<br />

System JOAN MARIE STEVENS, Gilson, Inc., Mark E Crawford, Luke<br />

Roenneburg<br />

4:05 (3020-4) Chiral HPLC Method Screening: Automated Method<br />

Screening Using a New Low Pressure Gradient Mixer with up to 24<br />

Independent Inlet Ports GARY W YANIK, PDR-Chiral<br />

ORAL SESSION Session 3030<br />

Ultrasensitive Detection in Bioanalysis (Half Session)<br />

Thursday Afternoon, Room 245<br />

Maria K Ferguson, PA Department of Environmental Protection, Presiding<br />

1:30 (3030-1) The Effects of Photon Shot Noise and Molecular<br />

Counting Error on Single Molecule Fluorescence Anisotropy SEAN<br />

BURROWS, Texas Tech University, Dimitri Pappas<br />

1:50 (3030-2) A Compact Single-Molecule Detection Instrument for<br />

Monitoring Point Mutations Using Single Pair FRET JASON M EMORY,<br />

Louisiana State University, Steven A Soper, Frederick Crawford-Drake<br />

2:10 (3030-3) Pattern-Recognition Nanopore Sensor for Single-<br />

Molecule Detection of Biomolecules QITAO ZHAO, University of Texas,<br />

Arlington, Deqiang Wang, Dilani A Jayawardhana, Xiyun Guan<br />

2:30 (3030-4) Pb 2+ Detection in Hybrid Nano/Microfluidic Device<br />

Utilizing DNAzymes Immobilized on Gold Coated Nano-capillary Array<br />

Membranes AIGARS PIRUSKA, University of Notre Dame du Lac, Yi Lu, Paul<br />

Bohn<br />

1:30 (3010-1) Impact of Stationary Phase Chemistry on Retention<br />

and Selectivity in Aqueous-Normal Phase/HILIC Separations DAVID S<br />

BELL, Supelco, Hillel Brandes, Craig Aurand<br />

1:50 (3010-2) Analysis of Metabolized Nucleoside Reverse<br />

Transcriptase Inhibitors through Ion-Exchange HPLC ANN M CAMPBELL,<br />

University at Buffalo, Melissa N Dunkle, Luis A Colon, Jill S Hochreiter, Gene D<br />

Morse<br />

2:10 (3010-3) A Comparison between On- and Off-line Heart-Cut<br />

Two-Dimensional LC for Impurity Profiling of Pharmaceutical Compounds<br />

SEBASTIAAN EELTINK, Dionex Corporation, Frank Steiner, Remco Swart<br />

2:30 (3010-4) Multi-Stage Sample Injection: An Effective Way to<br />

Reduce Band Broadening in Liquid Chromatography MARK HAYWARD,<br />

Lundbeck Research, QingPing Han<br />

2:50 Recess<br />

3:05 (3010-5) The Development of an Automated Forced<br />

Degradation and Method Validation Instrument ROY HELMY, Merck,<br />

Timothy Rhodes, Lina Liu, Wes Schafer, Elise Miller, Jia Zang, Margaret Figus,<br />

Bing Mao, Scott Donenfeld, Carlos Perez, Jim Caverly

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