LabChip GXII for High Throughput Analysis of Protein ... - PerkinElmer
LabChip GXII for High Throughput Analysis of Protein ... - PerkinElmer
LabChip GXII for High Throughput Analysis of Protein ... - PerkinElmer
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CE-SDS vs. <strong>LabChip</strong>: Non-Reducing mAb<br />
cence<br />
Fluores<br />
550<br />
40 sec<br />
Microchip-CE-SDS<br />
6<br />
500<br />
Migration Corrected Migration Corrected<br />
450<br />
Time, Relative Time, Relative<br />
400<br />
350<br />
Peak Number seconds Area minutes Area Delta<br />
300<br />
1 18.6 0.4% 15.5 0.7% 0.3%<br />
250<br />
200<br />
150<br />
100<br />
50<br />
0<br />
-50<br />
5<br />
1 2 3 4<br />
6<br />
7<br />
16 18 20 22 24 26 28 30 32<br />
Time (seconds)<br />
8<br />
5<br />
9<br />
0<br />
1<br />
LC-90<br />
CE-SDS<br />
2 20.5 0.1% 18.7 0.2% 0.1%<br />
3 23.9 0.3% 22.2 0.3% 0.0%<br />
4 26.4 0.5% 25.2 0.5% 0.0%<br />
5 27.8 2.0% 26.7 2.4% 0.4%<br />
6 29.0 96.7% 27.8 95.9% 9% 08% 0.8%<br />
Total Impurities 3.3% 4.1% 0.8%<br />
35 minute separation<br />
Baseline<br />
variability<br />
Data is quite consistent, despite<br />
differences in:<br />
• Instrument<br />
• Sample preparation buffer<br />
• Detection method<br />
• Some impurities are known to<br />
be sensitive to sample<br />
preparation conditions<br />
Siemiatkoski et al. Use <strong>of</strong> <strong>LabChip</strong> 90 in Product Development <strong>of</strong> a<br />
Recombinant Fusion <strong>Protein</strong>. <strong>Protein</strong> Summit 2008, London UK<br />
11
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