LabChip GXII for High Throughput Analysis of Protein ... - PerkinElmer

HT Glycan Assay Performance Specifications
8ul with concentration range of 1.25mg/ml to
Amount of sample required:
7.5mg/ml (10ug to 60ug of MAb total).
Reproducibility of %area:
CV < 10% for a peak >= 2.5% of total glycan.
1ng of G0F standard. (Smallest amount of labeled
Limit of detection:
G0f standard that can be detected.)
>95% of all N-Linked glycans will be released from
Deglycosylation:
mAb.
Appropriate for neutral glycans found on MAbs.
Usable size range: Some charged glycans may run outside of our
usable range.
Sizing ing reproducibility: CV < 2.5%
< 0.5% of previous sample can be detected in
Carryover:
following sample.
Sample prep, chip prep, and < 8 hours for a 96-well plate.
analysis time:
Number of samples per chip Up to 192 samples before chip needs to be
prep: reloaded with fresh gel.
Number of samples per chip Up to 384 samples before chip needs to be
lifetime: replaced.
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