LabChip GXII for High Throughput Analysis of Protein ... - PerkinElmer

Intermediate-Assay Reproducibility
•Labeled N-glycans released from three different mAbs at starting concentrations of 1.25 mg/mL, 2.5mg/mL, and 7.5
mg/mL were analyzed.
•The %RSD was calculated for 35 reactions (complete from deglycosylation to labeling and separation) per mAb, 2
repeat injections, 4 different chips and instruments, and 2 users.
•Total %RSD’s for the five main glycan peaks were all below 10% for peaks with relative amount greater than 2.5%.
IgG_A AVG % Area CV_10ug CV_20ug CV_60ug Total CV
G0 6.82 3.6% 3.4% 3.8% 6.3%
G0F 53.39 3.4% 1.6% 2.5% 3.2%
G1F 9.05 4.1% 1.9% 2.8% 4.1%
G1F' 9.28 4.6% 1.9% 2.1% 4.9%
G2F 2.05 12.2% 6.3% 2.2% 13.4%
Man5 4.61 4.9% 3.6% 6.3% 8.7%
IgG_B G0 10.55 5.8% 4.9% 4.2% 5.4%
G0F 47.78 2.4% 1.6% 1.2% 2.1%
G1F 5.70 5.1% 5.2% 4.3% 6.5%
G1F' 17.92 2.9% 2.0% 1.4% 3.2%
G2F 258 2.58 82% 8.2% 75% 7.5% 33% 3.3% 98% 9.8%
Man5 1.79 7.2% 8.8% 6.8% 12.1%
IgG_C G0 5.01 4.5% 3.2% 3.4% 6.8%
G0F 57.33 3.1% 2.1% 3.3% 3.1%
G1F 457 4.57 49% 4.9% 36% 3.6% 41% 4.1% 63% 6.3%
G1F' 13.92 3.5% 2.3% 1.8% 3.1%
G2F 1.25 14.4% 13.4% 6.7% 15.5%
Man5 5.58 4.9% 3.5% 3.3% 6.8%
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