LabChip GXII for High Throughput Analysis of Protein ... - PerkinElmer

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PNGase-F Digestion Observed complete deglycosylation • Digested IgG (red trace) shows shift towards NGHC when compared to undigested IgG (blue trace) HC NGHC 28
Use of Internal Standards for Peak Identifications • Individual neutral glycan standards show good resolution of the main glycans • The two G1F isomers, differing only in the branch position of terminal galactose, are resolved Mixture of Glycan Standards G1 29
Page 1 and 2: LabChip GXII for High Throughput An
Page 3 and 4: LabChip GXII Applications MicroChip
Page 5 and 6: Protein Assay Workflow Crude or pur
Page 7 and 8: (1) (3) (2) Resolution Ladder Mix (
Page 9 and 10: Protein Assay Specifications Type S
Page 11 and 12: CE-SDS vs. LabChip: Non-Reducing mA
Page 13 and 14: Analysis of Subunit Vaccine Express
Page 15 and 16: LabChip GX Dependent Process Develo
Page 17 and 18: High Sensitivity: Derivatization of
Page 19 and 20: Low Level Impurity Detection: Non-C
Page 21 and 22: Antibody N-linked Glycans Complex M
Page 23 and 24: IgG Charged N-Glycan Types Sialylat
Page 25 and 26: Goals for HT N-Glycan Screening Ass
Page 27: HT Glycan Assay Performance Specifi
Page 31 and 32: Use of Glucose Ladder For Peak Iden
Page 33 and 34: Intermediate-Assay Reproducibility
Page 35 and 36: Relative Amounts Comparison Glycan
Page 37 and 38: Acknowledgements Collaborators - Gr
Page 39: LabChip System Sampling of LabChip

LabChip GXII for High Throughput Analysis of Protein ... - PerkinElmer

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