Printable Test Catalog - OU Medicine

will be processed but not stained and evaluated
until the following Monday
Special arrangements must be made with the Viro
lab for specimens that must be obtained on
weekends after 12 PM Friday and before 12 PM on
Sunday; On holidays, specimens received before
10:00 AM will be processed but not stained and
evaluated until the following work day
REFERENCE RANGE: Negative
METHODOLOGY: Direct Fluorescent Antibody (Quantitation)
CLINICAL USE: Rapid diagnosis of cytomegalovirus
CPT: 87271
[VCMVFAI] CMV FA + CULTURE
SPECIMEN REQUIRED: Acceptable specimens: Bronchial/ respiratory
or biopsy material
NASOPHARYNGEAL WASH (NPW) Instructions:
1. Draw up 1-2 mL of sterile saline into a 3-5 mL syringe.
2. Remove the needle from the syringe and put on a butterfly
with a large tubing. Cut the tubing to a length of 2-3"
3. Place the tubing in the nasopharyngeal passage. Inject the
saline rapidly and draw back rapidly.
4. Inject saline and nasal material into the CHO viral transport
media; if viral transport medium is not available, NP was may
be added to 1.0 mL of trypticase soy broth or saline.
5. If immunofluorescent antibody (FA) testing is ordered after
or before doing th wash, use a Dacron NP swab to do a good
epithelial cell scraping of the nasoparyngeal area. Place
and cut the swab into the same vial as the wash.
(Immunofluorescent testing is done on epithelial cells).
Alternate FA Collection
1. Insert a type 1 NP swab into the back of the nasopharynx.
(Clear mucous from infants and children by aspirating the
mucous or NPW before performing specimen collection)*
2. Press down firmly and hold swab in place for one minute..
3. Remove the swab quickly to avoid drying and roll onto the
center of 2 slides. Keep smear to dime size on each slide.
4. Place slides in holder, label, seal, and transport.
The aspirate can be put in viral transport medium and used for
culture backup. Culture backup for adults requires a throat swab
or gargle placed in viral transport medium. If FA is positive
the culture is not performed. Select any 2 viruses to be tested
for, RSV, Parainfluenza virus, Influenza A/B, Adenovirus.
Influenza A and B can be tested for separately or pooled.
TUBE OR CONTAINER: Viral transport media
SPECIAL HANDLING: Transport immediately
TEST AVAILABILITY: Monday-Saturday
METHODOLOGY: Direct fluorescent antibody (DFA)
CPT: 87271; 87252
[VCMVPCR] CYTOMEGALOVIRUS PCR