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Printable Test Catalog - OU Medicine

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TURNAR<strong>OU</strong>ND TIME: 3 Days<br />

NORMAL TEST VALUES: Negative<br />

METHODOLOGY: Direct Fluorescent antibody (DFA)<br />

CLINICAL INFORMATION: Evaluate lower respiratory infections<br />

[VRSVFA5] RSV EIA/FA5<br />

[VRSVFA6] RSV EIA/FA6<br />

[VRSVPFA6] RSV EIA/PCR-FA 6<br />

[VRSVSVI] RSV SHELL VIAL + CULTURE<br />

SPECIMEN REQUIRED:<br />

Nasopharyngeal wash+NP instructions:<br />

1. Draw up 1-2 mL of sterile saline into a 3-5 mL syringe.<br />

2. Remove the needle from the syringe and put on a butterfly<br />

with a large tubing. Cut the tubing to a length of 2-3".<br />

3. Place the tubing in the nasopharyngeal passage. Inject the<br />

saline rapidly and draw back rapidly.<br />

4. Inject saline and nasal material into the viral transport<br />

medium. (If viral transport medium is not available, the NP+<br />

wash may be added to 1.O mL of trypticase soy broth or<br />

sterile saline.)<br />

5. After or before doing the wash, use a Dacron NP swab to do a<br />

good epithelial cell scraping of the nasopharyngeal area.<br />

Place and cut or break the swab into the same vial as the<br />

wash. Optimal testing requires epithelial cells. The<br />

combination of both the wash and nasopharyngeal scrapings<br />

are essential to obtain enough cells to perform the test.<br />

6. Transport Immediately<br />

TUBE OR CONTAINER: Viral transport media<br />

TEST AVAILABILITY: Monday-Saturday<br />

TURNAR<strong>OU</strong>ND TIEM: 48-96 hours<br />

METHODOLOGY: Shell Vial<br />

CPT: 87252; 87254<br />

[VRSVVC] RSV CULTURE<br />

SPECIMEN REQUIRED: Any respiratory specimen on ice<br />

COLLECTION INSTRUCTIONS:<br />

Nasopharyngeal wash+NP:<br />

1. Draw up 1-2 mL of sterile saline into a 3-5 mL syringe.<br />

2. Remove the needle from the syringe and put on a butterfly<br />

with a large tubing. Cut the tubing to a length of 2-3".<br />

3. Place the tubing in the nasopharyngeal passage. Inject the<br />

saline rapidly and draw back rapidly.<br />

4. Inject saline and nasal material into the viral transport<br />

medium. If viral transport medium is not available, the NP<br />

wash may be added to 1.O mL of trypticase soy broth or<br />

sterile saline.<br />

5. After or before doing the wash, use a dacron NP swab to do

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