QBI HISTOLOGY AND MICROSCOPY GUIDE
QBI HISTOLOGY AND MICROSCOPY GUIDE
QBI HISTOLOGY AND MICROSCOPY GUIDE
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14.Drain thoroughly of DAB before replacing in PBS to stop reaction<br />
15.Wash in PBS for 5 minutes (x3)<br />
16.To clean up DAB reaction, immerse plates in bleach then soak plates in detergent overnight. Wash<br />
throughly and rinse the water the following day<br />
17.Mount sections onto slides by floating in PBS. Allow at least 1 hour to dry.<br />
18. Haematoxylin Counterstain if necessary here.<br />
19.Dehydrate in the fume hood using the following sequence:<br />
1x2min washes of the following:dH20,75% EtOH; 95% EtOH.<br />
Followed by 2x2min washes in 100% EtOH,<br />
Then 3x2min washes in 100% Histolene (Xylene may be substituted here)<br />
20.Use DPX (xylene-based mounting media) to coverslip, leave in fume hood to dry overnight.<br />
21.Store slides in slide boxes at room temperature<br />
DAB stained neurons with<br />
Haemotoxylin counterstain<br />
20