Combinatorial Chemistry and Drug Discovery Lab Jasmine ... - haspi

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Combinatorial Chemistry and Drug Discovery Lab Student Protocol
Tips for handling E.coli: Lab Safety 1. Wipe down the lab bench or station with a 10% bleach solution or 70% isopropanol solution at the beginning and end of each laboratory session. 2. When creating mixtures and transferring chemical solutions or liquid bacterial cultures, keep nose and mouth away from the opening of the tube to avoid inhaling any aerosols that may be created. 3. All spills should be reported to your instructor and cleaned up immediately according to the Material Safety Data Sheets (MSDS) for each chemical used in the experiment. Be sure to wear proper footwear (closed toe) to prevent injury. 4. Dispose of any materials that have come in contact with bacterial cultures (i.e. tubes, pipettes) in special waste containers as provided d by your instructor. t 5. Wash hands with soap and water before leaving the lab.
Page 1 and 2: Combinatorial Chemistry and Drug Di
Page 3 and 4: Overview • In this lab students w
Page 5 and 6: National Science Education Standard
Page 7 and 8: Chemicals CHEMICAL VENDOR CATALOG #
Page 9 and 10: Presenter Notes Preparing Overnight
Page 11 and 12: Presenter Notes Spectrophotometer 1
Page 13: Instructor Notes If mixtures are no
Page 17 and 18: During the EXPONENTIAL or LOG PHASE
Page 19 and 20: Ouchterlony Test •A double diffus
Page 21 and 22: Label transfer pipettes 1. Label 6
Page 23 and 24: Label Petri dishes M1 M2 M4 M5 A#B1
Page 25 and 26: Prepare mixtures (libraries) 1. Mak
Page 27 and 28: Incubate at 37ºC or store at room
Page 29 and 30: Chemical Structures of Synthesized
Page 31 and 32: Data Analysis - + = no inhibition o
Page 33 and 34: Extension: Kirby-Bauer Test Determi
Page 35 and 36: Extension: Zone Size Interpretive C
Page 37 and 38: Results at Room Temperature
Page 39 and 40: Combinatorial Chemistry and Drug Di
Page 41 and 42: Three Major Classes of Antibacteria
Page 44 and 45: Antibacterial Sites of Action
Page 46: Great Job Jasmine Erfe!

Combinatorial Chemistry and Drug Discovery Lab Jasmine ... - haspi

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