Combinatorial Chemistry and Drug Discovery Lab Jasmine ... - haspi

More documents

Recommendations

Info

Data Analysis Mixtures (Libraries) M1 M2 M3 M4 A1-B1 A2-B1 A3-B1 M5 A1-B2 A2-B2 A3-B2 M6 A1-B3 A2-B3 A3-B3 Using this table, we can determine which compound in our active mixtures has antibiotic properties. First, shade in the column to indicate the mixture that shows antibiotic activity for compounds M1 thru M3. Next, shade in the row that corresponds to the mixture M4 thru M6 that shows antibiotic activity. The position in the table where the shaded column and shaded row intersect is the active compound.
Extension: Kirby–Bauer Test Determining Class Average Zone Size 1. After 24 hours in the incubator or 48 hours at room temperature check for the presence of antibiotic activity. This is done by looking for a clear area, called a zone of inhibition, surrounding a well. Remember to never open the Petri dish for a better view. Use the agar side to observe and measure any zone of inhibition. 2. Using a ruler, measure the diameter (in millimeters) of any zone of inhibition and record your individual group data on the data table provided. 3. Now gather class data to determine the average zones of inhibition, if present, for each of the different compounds M1 thru M6 and record the averages. 4. After recording the average class data for the diameters of the zones of inhibition, decide whether your groups sample of E.coli bacteria is susceptible (sensitive) displaying a CLEAR zone of inhibition, unaffected (resistant) showing no observable zone of inhibition or intermediate (somewhere in between) ee for each of the compounds M1 thru M6. Record your conclusion c o in the final column of the data table.
Page 1 and 2: Combinatorial Chemistry and Drug Di
Page 3 and 4: Overview • In this lab students w
Page 5 and 6: National Science Education Standard
Page 7 and 8: Chemicals CHEMICAL VENDOR CATALOG #
Page 9 and 10: Presenter Notes Preparing Overnight
Page 11 and 12: Presenter Notes Spectrophotometer 1
Page 13 and 14: Instructor Notes If mixtures are no
Page 15 and 16: Tips for handling E.coli: Lab Safet
Page 17 and 18: During the EXPONENTIAL or LOG PHASE
Page 19 and 20: Ouchterlony Test •A double diffus
Page 21 and 22: Label transfer pipettes 1. Label 6
Page 23 and 24: Label Petri dishes M1 M2 M4 M5 A#B1
Page 25 and 26: Prepare mixtures (libraries) 1. Mak
Page 27 and 28: Incubate at 37ºC or store at room
Page 29 and 30: Chemical Structures of Synthesized
Page 31: Data Analysis - + = no inhibition o
Page 35 and 36: Extension: Zone Size Interpretive C
Page 37 and 38: Results at Room Temperature
Page 39 and 40: Combinatorial Chemistry and Drug Di
Page 41 and 42: Three Major Classes of Antibacteria
Page 44 and 45: Antibacterial Sites of Action
Page 46: Great Job Jasmine Erfe!

Combinatorial Chemistry and Drug Discovery Lab Jasmine ... - haspi

Create successful ePaper yourself

Delete template?

Save as template?