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Combinatorial Chemistry and Drug Discovery Lab Jasmine ... - haspi

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During the<br />

EXPONENTIAL or LOG<br />

PHASE bacteria are<br />

growing <strong>and</strong> dividing<br />

at the maximal rate<br />

given their genetic<br />

potential, nature of the<br />

medium <strong>and</strong> conditions<br />

under which they are<br />

growing.<br />

In the STATIONARY<br />

PHASE, the total number<br />

of viable bacteria<br />

a<br />

remains constant. This<br />

may result from a<br />

balance between cell<br />

division <strong>and</strong> cell death or<br />

cells may cease to divide<br />

while remaining<br />

metabolically active.<br />

When microorganisms are<br />

introduced into fresh culture<br />

medium usually no immediate<br />

increase in cell number occurs<br />

therefore this is referred to as<br />

the LAG PHASE.<br />

Detrimental environmental<br />

conditions such as lack of<br />

nutrients <strong>and</strong> waste buildup<br />

lead to the decline, usually<br />

logarithmic, in the number of<br />

viable cells. This is characteristic<br />

of the DEATH PHASE.<br />

What limiting factors would cause a microbial<br />

population to enter the stationary phase?

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