Combinatorial Chemistry and Drug Discovery Lab Jasmine ... - haspi

Extension: Kirby–Bauer Test
Determining Class Average Zone Size
1. After 24 hours in the incubator or 48 hours at room temperature check for the
presence of antibiotic activity. This is done by looking for a clear area, called a
zone of inhibition, surrounding a well. Remember to never open the Petri dish
for a better view. Use the agar side to observe and measure any zone of
inhibition.
2. Using a ruler, measure the diameter (in millimeters) of any zone of inhibition and
record your individual group data on the data table provided.
3. Now gather class data to determine the average zones of inhibition, if present,
for each of the different compounds M1 thru M6 and record the averages.
4. After recording the average class data for the diameters of the zones of
inhibition, decide whether your groups sample of E.coli bacteria is susceptible
(sensitive) displaying a CLEAR zone of inhibition, unaffected (resistant)
showing no observable zone of inhibition or intermediate (somewhere in
between) ee for each of the compounds M1 thru M6. Record your conclusion c o in the
final column of the data table.