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Combinatorial Chemistry and Drug Discovery Lab Jasmine ... - haspi

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Prepare mixtures<br />

(libraries)<br />

1. Make sure that the<br />

proper transfer pipette is<br />

used for each solution to<br />

avoid contamination.<br />

2. Add solutions in the<br />

order indicated in the table.<br />

Record your observations.<br />

Tube # Add 5 drops Then 5 drops<br />

Then 5<br />

drops<br />

Then 15 drops<br />

M1 B1 B2 B3 A1<br />

M2 B1 B2 B3 A2<br />

NOTE:<br />

15 drops = ~ 750µL<br />

5 drops = ~ 250µL<br />

1 drop = .05 mL = 50 µL<br />

M3 B1 B2 B3 A3<br />

M4 A1 A2 A3 B1<br />

M5 A1 A2 A3 B2<br />

M6 A1 A2 A3 B3

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