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fusion of Brassica carinata BC1 x Brassica rapa subsp.
oleifera f. praecox 31/96. Preliminary flow cytometry
analyses showed a high rate of somatic hybrids (85%).
Further morphological and cytological analyses will be
performed after the subcultivation of regenerants to
greenhouse conditions. (Vyvadilová M., Klíma M.)
Sources of resistance and innovation
of selection methods for breeding for multiplex
resistance to the main wheat and oat leaf
diseases (MZe âR 1G58083)
The topic was investigated in cooperation with the
research centre SELTON. Resistance of wheat to the
following pathogens: Puccinia triticina, P. striiformis, P.
graminis, Blumeria graminis, Pyrenophora tritici-repentis,
Mycosphaerella graminicola and resistance of oats to
Puccinia coronata f.sp. avenae were studied. Ring tests
with single pathogens were carried out at several
locations. These tests enabled to determine multiplex
resistance. Resistance was also studied in greenhouse
tests on seedlings and on detached leaves. In these
tests resistance to rusts, Pyrenophora tritici-repentis and
Mycosphaerella graminicola was studied.
Pathotypes of wheat leaf rust and oat crown rust, as
well as of Mycosphaerella graminicola were studied and
total virulence of powdery mildew was determined on
a set of differentials.
The comparison of the greenhouse and field tests
enabled to distinguish resistance effective during the
whole ontogenesis from the adult plant resistance.
According to reactions of cultivars to a set of selected
pathotypes the translocation 1BL.1RS possessing
resistance genes Lr26, Sr31,Yr9 and Pm8 as well as the
translocation from Aegilops ventricosa carrying resistance
genes Lr 37, Sr38, Yr17 were postulated in several
registered winter wheat cultivars. (Hanzalová A.,
Barto‰ P., Dumalasová V.,Vûchet L., Klenová H.)
Genetic, physiologic and molecular analysis
of a novel flowering time gene in wheat
(GA âR 521/05/0257)
The development of plants of spring cultivar Zlatka
and of its substitution line Zlatka (âP3B) grown in the
short day (10 hours photoperiod, RICP shaded field
plots) was checked repeatedly to complete information
on the earliest stages where different dynamics of main
spike differentiation due to the chromosome 3B substitution
had been observed.
SSR molecular fingerprinting of the Zlatka (Zlatka
3B/ CP 3B) single chromosome recombinant F3 lines
was carried out to develop a further comprehensive
map of the chromosome 3B. The acquired data were
processed using computer software available at JIC to
develop a new genetic map of chromosome 3B and to
compare it with the previous map developed using the
Sandra (Sandra 3B/CP 3B) single chromosome recombinant
population map, and other 3B maps available at
the JIC based on the recombinant doubled haploid
populations of UK winter wheats Spark x Rialto and
Charger x Badger.The genetic maps were all co-linear
and indicated that the genetic maps available of the CP
3B chromosomes should provide sufficient coverage to
map the genes for flowering time and other traits.Two
markers were common between the Zlatka /CP 3B
and Sandra /CP 3B, which should also enable the
aligning of QTL data – see Figure. (Pánková K.,
Prá‰il I., DoleÏel J., Snape J. W.)
Advanced techniques in obtaining and
processing crops research results (Leonardo,
the project of mobility, CZ/06/A/PL/134148)
The substitution and recombinant substitution lines
which were the focus of the genetical analysis experiments
carried out by the trainee were derived from
a range of chromosome substitution lines developed in
RICP, Ruzynû, Prague. The first task was to monitor
the validity of historic collections maintained in RICP
through the use of molecular markers.The second task
was to carry out a large experiment to study variation
in the flowering time of F3 recombinant lines derived
from Sandra (CP 3B).An experiment under controlled
conditions was established in a controlled environment
room, and phenotypic analysis of flowering time and
other characters was carried out. Additionally, samples
were collected which were used for DNA extraction to
develop and check the genetic map of the 3B chromosome.
The genetic map and phenotypic data were
combined to carry out QTL analysis to locate quantitative
trait loci for flowering time and other traits.
(Pánková K., Milec Z., Snape J. W.)
Comparative
maps of wheat
chromosome 3B