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Longan production in Asia - United Nations in Indonesia

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Figure 11. ‘M<strong>in</strong>jiao No.1’ - an abort-seeded longan cultivar<br />

(from: Huang et al., 2000).<br />

In Ch<strong>in</strong>a, biotechnology research has achieved great progress <strong>in</strong> terms of its<br />

contribution to longan <strong>production</strong>, especially genetic improvement through modern high<br />

technology (Lai et al., 2000). Some of the biotechnology research <strong>in</strong>cludes tissue and<br />

organ culture, i.e., studies on plant regeneration obta<strong>in</strong>ed from explants such as<br />

cotyledons, immature embryos, anthers, leaves of bear<strong>in</strong>g trees, stem apices and stem<br />

axes. Plant regeneration has been successfully carried out via somatic embryogenesis<br />

from calli derived from the cotyledon of an immature embryo. A breakthrough was<br />

achieved <strong>in</strong> the <strong>in</strong>duction and long-term ma<strong>in</strong>tenance of friable embryogenic calli, and<br />

somatic embryogenesis at high frequency. S<strong>in</strong>gle cell clon<strong>in</strong>g technology and a highly<br />

effective protoplast regeneration system have been established from embryogenic<br />

suspension cell l<strong>in</strong>es by isolat<strong>in</strong>g s<strong>in</strong>gle cells and protoplasts. Agrobacterium-mediated<br />

genetic transformation has been established and transgenic plantlets of longan have been<br />

obta<strong>in</strong>ed (Zeng et al., 2000). Culture of shoot tips and shoot segments has been<br />

successfully carried out to obta<strong>in</strong> virus-free plantlets for overcom<strong>in</strong>g witches’ broom<br />

disease.<br />

26

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