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ImageJ Basics

ImageJ Basics

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Image type: greyscale LUTs, RGB<br />

‣Pseudocolour in greyscale:<br />

8bit and 12bit greyscale images can be visualised in colour:<br />

Use LookUp tables (LUTs): Assigns a range of colour to the grey levels<br />

> Either use LUT button on startup tool bar or switch to LUT tool bar by<br />

pressing on<br />

(Biophotonics edition)<br />

The following tool bar appears:<br />

‣Colour image (RGB):<br />

an overlap of 3 pixels 8bit (24 bit total)<br />

It’s an additive principle of colours:<br />

We can obtain a certain colour by adding 3 primary colours in various<br />

proportions: 256x256x256 = more than 16 million colours.<br />

Different type of colour coding: RGB, CYMK.<br />

RGB: each colour is coded on 1 octet (8bits). Each pixel is a combination of 3<br />

octets (3 x 8 = 24 bits total with Red = 0-255, Green = 0-255, Blue = 0-255.<br />

1 pixel = 3 octets = 24 bits<br />

Biology<br />

Imaging

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