ImageJ Basics

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Image information & Visualisation Status Bar = information for cursor position (x,y) and intensity value. Or memory usage Top of Image = information: Channels Image size (um or pixels) Image type (bits) Image memory Depending on image dimensions, Scroll bars appear at the bottom to scroll across c: channels z: stack slices t: time frames Biology Imaging
RGB channel splitting/merging/overlays ‣Image > Color > Merge (overlay)/Split channels Press once: Merges channels of an RGB stack to one RGB file Press twice: Splits into 3 individual channels Press again: Merges channels back with options as to which channel goes where. Overlay possible with transmitted light images by using the Gray channel. Do not create composite. Biology Imaging
Page 1 and 2: ImageJ Basics - open source public
Page 3 and 4: Installation Different editions of
Page 5 and 6: ImageJ memory ‣Set high memory fo
Page 7 and 8: File menu: Opening, importing, savi
Page 9 and 10: Image Menu Working with different i
Page 11: Image type: greyscale LUTs, RGB ‣
Page 15 and 16: …Stacks For immediate maximum int
Page 17 and 18: Image Adjustments: Threshold ‣Ima
Page 19 and 20: Calibrating image size units Calibr
Page 21 and 22: ImageJ Region measurements ‣ Area

ImageJ Basics

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