ImageJ Basics

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Plugins & Macros Plugins are additional software modules or code which provide the ability to perform specific tasks. Writing macros using the ImageJ macro language allows you to string a series of commands together to perform batch operations. These can also be converted to plugins. ‣Install plugins if required: Download plugins and Save them in ImageJ Plugin folder (program files) Restart ImageJ: Plugins automatically get placed in the Plugins menu > There is a list of available plugins here: http://rsb.info.nih.gov/ij/plugins/index.html > There is a plugins collection preorganised in folders available at: http://rsb.info.nih.gov/ij/plugins/collection.html > Many more are available on the website and from other users. Some people have developed their own collections of plugins and bundled them together. McMaster Biophotonics Institute has a great collection for microscopy: http://rsb.info.nih.gov/ij/plugins/mbf-collection.html Biology Imaging
ImageJ memory ‣Set high memory for optimal use no more than 70% of max Ram ‣Edit > Options > Memory Biology Imaging
Page 1 and 2: ImageJ Basics - open source public
Page 3: Installation Different editions of
Page 7 and 8: File menu: Opening, importing, savi
Page 9 and 10: Image Menu Working with different i
Page 11 and 12: Image type: greyscale LUTs, RGB ‣
Page 13 and 14: RGB channel splitting/merging/overl
Page 15 and 16: …Stacks For immediate maximum int
Page 17 and 18: Image Adjustments: Threshold ‣Ima
Page 19 and 20: Calibrating image size units Calibr
Page 21 and 22: ImageJ Region measurements ‣ Area

ImageJ Basics

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