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equivalent). The latter can be used directly with a sterile vacuum filter unit if not using
syringe filtration.
6.5 SAMPLE FILTERS - Sterile, single-use, disposable surfactant-free cellulose acetate
(SFCA) 26 mm, 0.2 µm syringe filter (Fisher Scientific, Suwanee, GA, Corning Brand,
Part No. 09-754-13 or equivalent). For samples high in particulates, filters with built-in
prefilters are available. All samples must be filtered at the time of sample collection.
6.6 SYRINGES - Sterile, single-use, disposable, silicone-free, luer-lok, 20 mL (Fisher
Scientific, Suwanee, GA, Target Brand, Part No. 03-377-30 or equivalent).
6.7 SAMPLE PRETREATMENT CARTRIDGES - Single-use, disposable OnGuard-II H
cartridges (Dionex, Part No. 057085 or equivalent) used to remove high concentrations
of carbonate if it is determined to be an interferant. OnGuard-II Ba 2+ cartridges
(Dionex, Part No. 57093 or equivalent) used to remove high concentrations of sulfate if
it is determined to be an interferant. OnGuard-II Ag cartridges (Dionex, Part No. 57089
or equivalent) used to remove high concentrations of chloride if it is determined to be an
interferant. The Ba 2+ pretreatment cartridge is the only one that may be required to meet
the QC requirements of this method (Sect.11.6.2)
6.8 MICRO-PIPETTES - 250 µL, 1000 µL and 10 mL sizes with single-use disposable tips
(Rainin, Oakland, CA, Part Nos. EP-250, EP-1000, and EP-10 mL or equivalent).
6.9 VIALS - Single use, disposable autosampler vials with filter caps, or other disposable,
single use vials with caps having a 10 mL or less capacity to be used for sample
preparation.
7. REAGENTS AND STANDARDS
7.1 REAGENTS AND SOLVENTS - Reagent grade or better chemicals should be used.
Unless otherwise indicated, it is intended that all reagents shall conform to the
specifications of the Committee on Analytical Reagents of the American Chemical
Society (ACS), where such specifications are available. Solvents should be HPLC
grade or better. Other grades may be used provided it is first determined that the reagent
is of sufficiently high purity to permit its use without lessening the quality of the
determination.
7.1.1 HIGH PURITY REAGENT WATER (RW) - Purified water which does not
contain any measurable quantity of the target analyte or interfering compounds
at concentrations > 1/3 the MRL for the target analyte. The purity of the water
required for this method cannot be overly emphasized. For this work, deionized
water was further purified using a bench model Millipore water purification
system (Millipore Corp, Billerica, MA, Model No. MilliQ Gradient A10 or
equivalent).
332.0-12