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Nitrazine Paper - Point of Care Testing - Duke University

Nitrazine Paper - Point of Care Testing - Duke University

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Page 2 <strong>of</strong> 3<br />

<strong>Nitrazine</strong> pH analysis must be ordered by the patient’s physician.<br />

PATIENT IDENTIFICATION:<br />

Verify patient identification by checking the patient's name and date <strong>of</strong> birth on the arm band.<br />

PATIENT PREPARATION:<br />

Explain the purpose <strong>of</strong> the test and the steps <strong>of</strong> the procedure to the patient prior to performing<br />

the test.<br />

The operator’s hands must be washed before and after testing.<br />

Because <strong>of</strong> the hazardous nature <strong>of</strong> handling specimens contaminated with blood and body<br />

fluids, it is required that disposable latex gloves or an acceptable substitute be used when<br />

collecting specimens and test procedures. Standard precautions shall be observed for all<br />

specimens contaminated with blood and body fluid and they should be handled at Biosafety<br />

Level 2 as recommended for any potentially infectious material in the Centers for Disease<br />

Control/National Institutes <strong>of</strong> Health manual, Biosafety in Microbiological and Biomedical<br />

Laboratories, 1988 or in the National Committee for Clinical Laboratory Standard Document<br />

M29, Protection <strong>of</strong> Laboratory Workers from Infectious Disease Transmitted by Blood, Body<br />

Fluids, and Tissue, 1991.<br />

SUPPLIES: Gloves<br />

<strong>Nitrazine</strong> paper<br />

<strong>Nitrazine</strong> pH Quality Control buffers<br />

STORAGE:<br />

It is recommended that the <strong>Nitrazine</strong> pH paper be stored securely in its holder in a dry place at room<br />

temperature. <strong>Nitrazine</strong> pH papers expire on the date printed on the foil wrapper.<br />

QUALITY CONTROL:<br />

1. <strong>Nitrazine</strong> pH paper test strips should be tested when opening a new container and at least<br />

weekly using <strong>Nitrazine</strong> pH buffer controls, 5.0 and 7.0.<br />

2. Check the expiration date <strong>of</strong> the <strong>Nitrazine</strong> pH paper.<br />

3. Check the pH <strong>of</strong> each buffer; 5.0 and 7.0. Record the results on the <strong>Nitrazine</strong> pH QC Log.<br />

4. Note any failures on the <strong>Nitrazine</strong> pH QC Log. Retest; if failure again, obtain a new box <strong>of</strong><br />

<strong>Nitrazine</strong> pH paper and /or buffer(s) and follow steps= 1-4. Document corrective action<br />

taken on the QC Log.<br />

PROCEDURE:<br />

1. Explain procedure to woman/couple.<br />

2. Ensure that the fluid is appropriate for <strong>Nitrazine</strong> pH testing. Do not use for urine or gastric<br />

pH.<br />

3. Check the expiration date <strong>of</strong> the <strong>Nitrazine</strong> pH paper.<br />

4. Remove 3-4 inches <strong>of</strong> <strong>Nitrazine</strong> pH paper from container. With gloved hand, insert<br />

<strong>Nitrazine</strong> pH paper into the introitus <strong>of</strong> the vagina. Remove hand and paper.

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