Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
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Matrix preparation on the MALDI-MS targets<br />
Analysis of glycoproteins using 2,5-DHAP<br />
For preparation of glycoproteins within the mass range of 6 – 100 kDa on AnchorChip TM 600<br />
targets (#209513) we recommend the use of 2,5-dihydroxyacetophenone (2,5-DHAP, #231829 or<br />
#231830) as matrix solution.<br />
2,5-DHAP preparation on AnchorChip600 targets<br />
1. Dissolve 7.6 mg DHAP in 375 µl<br />
ethanol.<br />
: DHAP is irritant (R: 36/37/38; S:<br />
26-28-36/37-60), ethanol is highly<br />
flammable (R: 11; S: (2)-7-16)<br />
7.6 mg DHAP<br />
375 µl ethanol<br />
2. Add 125 µl (18 mg/ml in Milli-Q water)<br />
of a di-ammonium hydrogencitrate<br />
solution (= 10 µmol).<br />
: Diammonium hydrogencitrate is<br />
irritant (R: 36/37; S: 26)<br />
125 µl Diammonium<br />
Hydrogen Citrate<br />
3. Vortex <strong>for</strong> 1 min at RT. Vortex<br />
4. Sonicate <strong>for</strong> 15 min. Sonificate<br />
5. Vortex <strong>for</strong> 1 min at RT. The matrix<br />
solution is stable <strong>for</strong> at least one week<br />
when kept in the dark<br />
Vortex<br />
6. Mix 2 µl of the sample with 2 µl 2 %<br />
TFA and subsequently add 2 µl matrix<br />
solution.<br />
: 2% TFA is irritant (R: 36/38; S: 26-<br />
28-37-60)<br />
2 µl Sample<br />
2 µl 2% TFA<br />
2 µl DHAP Matrix Solution<br />
7. The mixture of sample, TFA and DHAP<br />
has to be vigorously mixed by pipetting<br />
up and down. It is important that the<br />
solution becomes turbid be<strong>for</strong>e spotting<br />
Vigorously mixing<br />
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